The Uterine Bacteria In Both Postpartum Normal And Endometritis Affected Cows

The bacterial flera, the number of bacteria and the sensitively of the bacteria to seneral antibiotics were studied in both normal cows (No=5) and the cows with endometritis (No=6) postpartum through uterine sample, taken by biopsy needle of the endometrium. The results showed as follows: All uterus of the experimental cows had isolate immediately afrer calving, which changes in their number and species with the postpartum time. By day 2 postpartum the number of the bacteria increased rapidly to (1 .91?.44)x 105/mg uterine contents (UC). During the days 2? postpartum the bacteria increased in number slowly and reached reached (3.93.61)x 106/mg UC on the day 7 postpartum. Then the number decreased slowly. The mean number of bacteria to (3 .40?.94)x 103/mg UC and (2.49?.92)x l02/mg UC by day 14 and 24 postpartum, respectively, and by day 39 postpartum, the mean number of bacteria was below lx 10/mg UC. There were an average of 3.4 bacterial species during days 2 to 7 postpartum and by days 14,24 and 39 postpartum it were on the average of 2.4, 1.2 and 1, respectively. The mean number of newly increased bacterial species was 3.4 within 2 days postpartum, and were 1.4, 0.6,0.4 and 0.4 by days 7,14 and 24 and 39 postpartum, respectively. With an exception of staphylococcus, there was no continuity for any other bacteria in their appearance and disappearance from the uterus. The bacteria species in the uterus during involution periods included staphylococcus, Eschetichia coil, Bacillus and Streptococcus in both normal cows and the cows with endometritis there were 91.7% of bacteria isolated were hemolytic compared with 28.1% hemolytic isolated from the normal cows. All the five normal cows had their uterine involution completed within 39 days postpartum. All the bacteria isolated from both normal cows and cows with endometritis were most sensitive to ciprofloxacin, enrofloxacin cefaznlin, and sensitive to the combination use of penicillin and slreptomycin. However, there were not sensitive to Shuang huanglian, aminobenzylpenicilin and tetracycline. The above results indicate that the species and the number of bacteria in the postpartum uterus of the cow experienced a shifting process. Bacteria can invade uterus during the whole period of involution, however, there is no notable effect in the rate of uterine involution. There are conditional pathogens in the normal uterus of the postpartum cow which can induce endometzitis. Also it was suggested in the experi- ment that the best time for clearing uterine bacteria is on the days 6 to 8 postpartum, and ciprofloxacin, enrofloxacin and cefazolin can be used for the clearance. Postgraduate: Wang Guoqing Major : Veterinary medicine Supervisor: Pmfessor Turn Wenm...