The Molecular Genetic Marker Of Different Eel Species' Populations Genetic Structure And Quality Evaluation

Random amplified polymorphism DNA (RAPD) technique was used to analyses the genetic structure and quality evaluation of three eel species. The feasibility of random amplified polymorphism DNA (RAPD) technique in the genetic breeding of fishery was also investigated. In all 32 random primers, 20 random primers were chosen and used to amplify the individuals?DNA of three eel species. The 20 primers all generated amplification bands, whose numbers ranged from I to 13 and sizes were from 500 to 2300bp.In 32 random primers, there are altogether 14 primers were chosen in which 37 special genetic marker were included. By using of the primers S2~. Si 522% Sz5~ Sri~ 52:.. 529.. 530.. and S43, Japanese eel ,European eel and American eel can be identified and 2 of 3 eel species can be identified with the using of the primers of 53.. S6.. S24 and S 112. The within population ~x siuuhuity c~f three eel species respectively is that: Japanese eel is 0.885; European eel is 0.717 and American eel is 0.686. The similarity index between Japanese eel and European eel is 0.557; which between Japanese eel and American eel is 0.582 and which between European eel and American eel is 0.880. 1 The genetic distance statistics of three eel species are: between Japanese eel and European eel is 0.747; between Japanese eel and American eel is 0.668 and between European eel and American eel is 0.2 11. The statistics of all above shows that RAPD technique can be used to evaluate fishery quality and sure the relationship of different populations.