Identification Of Reticuloendotheliosis Virus And Sequence Analysis Of Its Long Terminal Region

A pair of primers was synthesized according to the reported sequence of Long TerminalRepeats(LTR) for Reticuloendotheliosis Virus(REV). LTR fragment of REV was amplified by Polymerase Chain Reaction(PCR) from gross DNA which were extracted from spleens and livers of flocks suspected infected by REV, The results showed 21 samples shows positive in all the 56 samples. Four PCR positive samples from Jiangsu(2 samples), Shandong and Shanghai were cloned into TA vector and sequenced. Sequence analysis showed that the the homology is higher than 94%,and two sequences are the same.The same samples to PCR were also used to indentify REV in dot-blot test, with preREV genomic cDNA with digoxin labled probe. The result showed PCR was more sensitive than dot-blot and IIFA. All positive samples were 21 in all 56 samples were positive in dot-blot test except sample 31.REV was isolated with Chicken Embryonic Firoblast(CEF) from the PCR positive samples. The results indicated that 15 samples were positive to Monoclone antibody to REV in Indirect Fluorescent Assay(IFA). All 15 positive samples were positive in PCR and dot-hybridization.