Studies On The Relationship Between Polymorphism Of Porcine Growth Hormone Gene And Their Production Performance

The genetic polymorphisms of porcine growth hormone (J)GH) gene from ? 19-.-+486 amplified by PCR were characterized with RFLPs in five pig breeds, which were Jiangquhai ~. Xiaomeishan Duroc~ Pietrain and Landrace. The polymorphism restriction sites for Bsp I were identified at +193, two for Hha I at +330 and +379. The genotypes and allele frequencies in every polymorphism restriction sites were calculated in every breed, and the differences in genotype and allele frequencies in five breeds were tested by X2 . The effect of them on live-weight and live-weight-increase was analyzed. The results showed that the difference in genotype frequencies at Bsp I and Hha I restriction sites in five breeds was extremely significant respectively (P<0. 01). The highest allele frequencies at Bsp I restriction sites was A2 in every breed. But the allele (C1~ C2 ~. C4) frequencies at Hha I restriction sites were similar, which was about 30%. There was no difference of live-weight and live-weight-increase between genotype individual at Bsp I restriction sites in Jiangquhai swine herd. At Hha I polymorphism restriction sites, the 20-day-live-weight and 70-day-live- weight of C1C2 genotype individual was significantly higher than that of CC4 genotype individual (P<0. 05), the live-weight-increase between newborn and 20 days of C1C2 genotype individual was significantly higher than that of C1C4 genotype individual, the same that C1C2 genotype individual was significantly higher than C2C4 genotype individual between 45 days and 70 days (P<0. 05). There was no significance of newborn litter weight between Bsp I genotype individual, and the same result was got between Hha I genotype individual.