Polymorphisms Of POU1F1 Gene And Correlative Analysis With Weight Traits Of Liangshan Semi-wool Sheep

POU1F1 is the first certified pituitary transcription factor, it plays a role in control ofgrowth process and metabolism, it has far-ranging biological functions in improvinggrowth, reducing fat aggradation and so on. Six primers (P1, P2, P3, P4, P5, P6) weredesigned according exon 2, exon 3, exon 5 and intron 4 of sheep POU1F1 gene.Corresponding sequence of LS, ZS, NBG, JDG, JBG, and CMG popuLations were ampliedby PCR. Polymorphisms of POU1F1 gene were analyzed by PCR-SSCP and alsocorrelative analysis between polymorphisms and weight (BW, WW, WDG, 18W, 30W)traits of LS.In all designed primers, amplified fragment of primer P2(exon 3) have porlymorphismsin all six studied popuLations(LS, ZS, JDG, JBG, CMG, NBG), polymorphisms of primerP4 and P5 fragment only exist in LS sheep population. There were no polymorphisms inprimer P1, P3, P6 PCR fragment in all six studied populations. Primer 2 PCR fragment hasthree genotypes in LS, JDG, JBG and NBG populations, it was defined as EE, DE and EE,genotype DD was dominant in this locus of all six studied popuLations; Primer 4 fragmentalso had three genotypes in LS populations, it was defined as AA, AB, and AC, thefrequencies of genotype AA, AB, AC and allele A, B, C were 0.6082, 0.268, 0.1237/0.8041,0.1340, 0.0619 respectively, genotype AA was dominant; Only two genotypes weredetected in Primer P5 PCR fragment, it was defined as GG, FF, the frequencies of genotypeGG, FF and allele G/F were 0.9, 0.1/0.9, 0.1 respectively, genotype GG was dominant.Results of Genetic characteristics were that there were low polymorphisms on primerP2 fragment in LS, JBG and JDG populations (PIC 0.1645, 0.2433, 0.2442 respectively),midrange polymorphisms exists in ZS, CMG, and NBG populations (PIC 0.3192, 0.3671,0.347 respectively); primer P4 fragment also midrange polymorphisms in LS sheeppopulations (PIC=0.303), primer P5 fragment low polymorphisms in LS sheep populations(PIC=0.164).Results of correlative analysis of phenotype weight traits were that birth weight and18-month weight (r=0.5408, P＜0.01), Weaning weight and Weaning daily gain (r=0.9784, P＜0.01) had great significant difference. Results of variance analysis were thatgenotype had significant difference and great significant difference to birth weight and 18-month weight respectively in primer 2 of LS sheep; family effect had significantdifference and great significant difference to birth weight and 18-month weightrespectively in primer 4; year effect of three polymorphism primers had great significantdifference to birth weight, 18-month weight, and 30-month weight. Comparisons of leastsquare was carried between different genotypes and birth weight, 18-month weight ofprimer 2 PCR fragment, the results were that influence of genotype DE on birth weightsignificant exceeded genotype DD, but genotype DE had great significant difference withgenotype DD and EE in 18-month weight.