Quantatitive And Qualitative Assay Of BF's Extracts And Immunopotentiating Effect Of BF's Extracts On Mice And Piglet

BF抯 extracts which cotain many biological active elements, especially some little peptides such as tripeptides.. tetrapeptide etc, can promote the differentiation and maturation of the B lymphocyte of birds and mammals0 Many researchers have reported that BF抯 extracts can increase the number of mouse PFC enhance the cAMP and cGMP level of cell 0 In addition, some researchers have isolated bursin-tripeptide from the intrahepatic bile ducts and bone marrow of mammals0 However, there are many empty margins in the research such as the composition of BF抯 extracts.. tripeptide quantity of BF抯 extracts.. immunopotentiating effects of BF抯 extracts on mouse and piglet and feasible research as immunopetentiator for mammals 0In experiment(l), BF抯 extracts were assayed with Thin Layer Chromagraph and Thin Layer Scanning0 The results indicated that BF抯 extracts (IKD)have nine sorts of little peptides or amone acids0In experiment(2), Several immunological parameters including organ indexs number of WBCquantity of lymphocytes~ number of ANAE~ lymphocyte Ratio of ANAE~ lymphocyte to lymphocyte.. PRV antibody level, histological change of spleen.. challenge results of mice postinoculated with different doses of BF抯extracts and PRV inactive vaccine were measured to learn effects of BFs extracts on mouse immunity0 These results indicated that some doses of BF s extracts can inhance the mouse immunity in different degree; but some doses of BFs extracts can put negative effect on the mouse immunity0 At same time, these effects are related to dosage~. route and inoculative time In experiment(3). Several immunological parameters including PRV antibody level (VN.. ELISA).. Sera IgG quantity of piglets postinoculated with different doses of BFs extracts and PRV inactive vaccine were measured to learn the immunopotentiating effect of BFs extracts on piglet0 These results indicated that some doses (1. 2. 5mg per animal) of BF s extracts can inhance the piglet immunity in different degree; but some doses (10~ 25mg per animal) of BF s extracts can put negative effect on the piglet immunity0 At same time, these effects are related to dosage.. inoculative time In experiment(4), Several growth parameters includingWeight Gain per day.. Ratio of food to meat of piglets postinoculated with different doses of2BF s extracts and PRV inactive are measured to learn effects of BFs extracts on piglets growth0 These results indicated that some doses of BF s extracts can inhance the piglet growth in different degree; but some doses of ElF s extracts can put negative effect on the piglets growth 0 At same time,these effects are related to dosage~ inoculative time0...