The Progesterone Effects On The Immune Condition Of Uterus

The objective of this study is to investigate the progesterone effects on the immune condition of mice uterus. During the study, medicines and chemical reagents were given at 7:00 p.m.in day 5 or day 9 of gestation.CDmifepristone was given to block the biding of progesterone to its recepters;(2)salin was given;(3)progesterone was given;(4)the Chinese herbal medicine prescription composed of Radix scutellariae and Rhizoma atractylodis was given at day3,day 4,day5,then mifeprestone was given at day 5;(5)progesterone was given at 8h after the mifeprestone had been given. Three mice were killed at 4h,8h,12h,16h or 24h after 7:00 p.m. at day 5 or day 9 of gestation every group. Uterine NK cells and CD8+ T lymphocyte were analyzed by immunohistochemistry and IFN- Y and TNF- a contents were analyzed by Enzyme Linked Immunosorbent Assay (ELISA), respectively. After the mifeprestone had been given at day 5 of gestation, IFN- Y content increased (P<0.01),and the number of NK cells increased significantly ;at day 9 of gestation , IFN- Y and TNF- a content increased(P<0.05) respectively, the number of NK cells increased significantly (PO.05), but less CD8+ T lymphocytes were counted(P<0.05).Progesterone was given at day 5 of gestation, IFN- Y content decreased significantly (PO.01), more NK cells were counted and more CD8+ T lymphocytes were counted (PO.01) in the uterine Muscle; while at day 9 of gestation, IFN- Y content increased significantly(P<0.05),less NK cells were counted(P<0.01), and less CD8+T lymphocytes were counted (P<0.01) in the endometria and muscle of the uterus .In the herb prevention group, at day 5 IFN-Y increased, less CD8+ T cells were counted in endometria and muscle of the uterus (PO.01 ) ; At day 9, IFN-Y content and the number of NK cells increased significantly(PO.01),less CD8+ T lymphocytes were counted (PO.01), but IFN-Y and TNF- a contents had been intent to decrease at 4h. the result therefore indicate that progesterone of the Physiologic content inhibits the extreme increase of IFN- Y andTNF- a contents in the uterus .That mifepristone was given to block the immune condition develops to Thl-type response .So that IFN-y content increased in the uterus, NK cells gather and infiltrate, those induce the embryo loss. And the effect on the abortion at day 9 of gestation is more obvious than that at day 5.In the progress of normal pregnancy, the NK cells, IFN- v and TNF- a in the endometria benefit to the embedding of embryo and the maintence of pregnancy, progesterone may increase the number of NK cells and CD8+T lymphocytes at day 5 of gestation. Though progesterone and the Chinese herbal medicine prescription composed of Radix scutellariae and Rhizoma atractylodis couldn't entirely prevent the mifeprestone-induced abortion.that have a certain antiabortive effect.