Study On Screening Of Cellulase Producing Strain For Enzymatic Feed And Property Of Cellulase

A strain Trichoderma koningii No.0143 which produces cellulase was isolated from a soil sample of the east area in QingHai, its FPA activity was 15u/g. After treatment No.0143 strain with NTG, a mutant (No.0143p) was obtained whose FPA activity was improved from 15u/g to 20u/g. After selected carbon and nitrogen resources, the incubated time, pH and temperature, the FPA activity was improved further to 32 FPA units. It was proved that wheat bran, urea, pH 7.0, 28℃It was showed that No.0143p strain was promising fungi in enzymatic feed. The cellulase optimum PH and temperature were 4.8. and 55℃ respectively.