Study On Genetic Relationship And Physiological And Biochemical Parameters In Different Pigeonpea Varieties

Five different pigeonpea varieties from different areas (i.e. Guangxi, Hainan, Africa, Burma and India ) and three hybrids bred by ICRISAT were investigated. Pn, Chi and MDA contents, and the ratio of bound-water/free-water were determined. The biological characters were investigated in the field. And the biological morphological markers, peroxidase isozymes and RAPD techniques were used for clustering the varieties.The results showed as follows:1. Pn and Chi contents in ICPL87091 were higher than any other varieties remarkably. It indicated a considerable photosynthetic capacity in ICPL87091. The following was the Guangxi variety.2. The ratio of bound-water/free-water in Burma, Guangxi and Indian varieties were higher than any others, but the content of MDA in these varieties was lower than 5 other varieties. It showed that the drought resistance in these varieties was stronger than any other varieties. However, the Hainan variety showed opposite.3. The result of peroxidase isozyme showed that the numbers of electrophoresis profile and the moving distance were the same in 8 different varieties, except for the color deepness in the profiles.4. Base on the observation on the 8 pigeonpea varieties for one year, some differences were detected among these varieties, such as growth duration, flower color and seed color etc. The results of cluster analysis in 18 quantity characters demonstrated that there was a high proximity between varieties. The cluster analysis of RAPD data showed the similar results, and the average genetic distance is 0.25. It further proved that they had a narrow genetic basis, and showed a close genetic relationship.