Study On Genetic Stability Of Rumexk-1(Rumex Patientia×R.tianschanicus,cv.Rumexk-1) | Posted on:2003-01-06 | Degree:Master | Type:Thesis | Country:China | Candidate:Y L Zhang | Full Text:PDF | GTID:2133360062995007 | Subject:Works of Prataculturae | Abstract/Summary: | PDF Full Text Request | Study on Genetic Stability of Rumexkï¼l (Rumex patlentiaxR.tlanschanlcus,cv.Rumexkï¼l) Rumexkï¼l(Rume patientia L.XR.tianschanicus A.Los,cv.Rumexkï¼l) Is sort ofa new highï¼protein plant by distant hybrldlzatlon ofRumex patientla x R.tlanschanlcus.To determine whether variety traits are stability,the experienced study on the varletygenetlc stability In all level,Including genetic stability of*theity agronomy traits,characters correlation,crude protein contents and tannin contents In different position In bud stage and oxalate contents In bud and leafcluster stage, genetic diversity analysis and so on,the results of*thes experiment are as follows: IThe result ofgenetlc stability analysis showsthatthe agronomy traits,such as length of lea二 width of leaå°º length of stem, andlengthï¼wldthratloï¼›hayï¼freshratlo andstemï¼leafratlo Inbud stage,grass yield,speed of regrowth,the Intensity of tuning green and days from sowingtobearingare stable Inthree continued generations.There were no significant differences amongthe three contlnuedgeneratlons Inthese agronomytraltsbythe oneï¼factor variance analysis andFï¼test. 2 There Is no variance onthe traits ofplantheight,length of Inflorescence,branches number,fertility and KGW,branches number OffOIC illnOfCSCCflCC,ylCld pCT p18llt,fOIC lfinoæ£CSCCllCC hClght &lid lowest Inflorescence heightbythe variance analysis andFï¼test,which means It has genetic stability.Correlation analyse shows thatbranchers number and branches number ofrole Inflorescence are strongposltlvely related with yield per plant In the three continued generations ofRumexkï¼l,dlrectpathanalyse showsthatdlrectï¼effectoflengthof role Inflorescence and brachers numberic yleldperplant Is laregest,bymeasuring oflenghth ofinflorescence and branchers numberic forecastseed yield. 3 Crudeprotelncontentofthethree contlnuedpeneratlons Inbud stage Is as follows respectively:breeder seed.52ï¼…,fundatlonseed 15.58ï¼…,certification seed 15.37%.There Is no difference Incontents,which means It Is genetic stable. 4 Tannin content In dlå¾rent position leafls as followsrespectively:breeder seed 0.2091ï¼…and fundatlon seed 0.1740ï¼…andcertification seed 0.1862ï¼…,stem Is asfollows respectively:breederseed 0ï¼…and刊ndaton seed 0.01扔%and certncatlon seed 0.皿033ï¼…,Inflorescence Is as follows respectively:breeder seed 2.261ï¼…andfundatlon seedZ.220ï¼…andcertlficatlon seedZ.300%.varianceanalysis showsthatlt Is genetic stable Inthethree continuedgenerations. 5 Oxalate contents In bud and leaåœcluster stage.all plant Inbudstage Is as follows respectively:breeder seed 2.069ï¼…and fundationseed 2.059ï¼…and certlncatlon seed.956ï¼…,leafï¼cluster stage Is asfollows respectively:breeder seed 4.350ï¼…and fundation seed 4.353ï¼…and certification seed 4.474%.there Is no slgnlhcant difference amongthe three continued generations In oxalate contents. 6 Thegenetlcploymorphlsms InRumexkï¼lwas Investigatedusingrandom ampllhedploymorphlc DNAmarkers,the detectedmaterials Includedthlrteen spedes,sevenprlmers selectedfrom twentyprimers amplltled 45 RAPD fragments,the length of most amplifiedfragments rangedfrom300to2500bp.theRAPDresultshowthatthereIs no diversity amongthe three continued generations.The geneticstabllltyls goodlnthethree continued generations ofRumexkï¼1.Butthere Is some difference onRAPD characteristic markers In 12perplantof certification seed. ByRAPD fragments andthe percentage ofpolymorphlc loci can prellmlnarydetermlne that Rumex...
| Keywords/Search Tags: | Rumexk-1(Rumex patientiaXR.tianschanicus,cv.Rumexk-1), Genetic stability, Protein, Tannin, Oxalate, RAPD technology | PDF Full Text Request | Related items |
| |
|