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Study On Genetic Characteristics In Rumex K-1(Rumex Patientia ×R.tianschanicus ,cv.Rumex K-1)

Posted on:2002-04-21Degree:MasterType:Thesis
Country:ChinaCandidate:X F JuFull Text:PDF
GTID:2133360032955802Subject:Grassland
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Rumex k-1( Rumex patientia ×R.tianschanicus , cv. Rumex K-1 )is a new high-protein forage variety by distant hybridization of Rumex patientia ×R.tianschanicus .It has the features of high production and good quality and wide adaptability so that Rumex K-1 has been introduced to all over the country,but there are several existing problems such as weather variety traits are stability and the variance change yield and quality . The key to these problems is to establish the scientific seed production system .Thereby, it is very important to do the research of the variety genetic stability ,the manner of pollination and reproduction , cytology , which will have important theoretical and practical significances to establish seed production system , seed production and extension . The results of the experiment are as follows: 1. The genetic stability analysis has shown the agronomy traits, such as leaf length, width and length/width ratio, growing stage, hay ratio,yield, regrowth speed, contents of crude protein and water, were stable in three continuous generations. Moreover the statistical one-factor square variance and F-test displayed that there were no significant differences among the three continued generations in these agronomy traits. Leaf length/width ratio was strong positively related with regrowth speed. 2. Through the analyzing of seed storage protein and enzyme by using SDS-PAGE AND PAGE , the results showed that the three continued generations of rumex k-1 have the same stroage protein and POD pattern. There is no variance in those traits , which means it has genetic stability. 3. Chromosome numbers were counted in cells of root tips of rumex k-1, Rumex patientia L., R.tianschanicus A.LDS. The result showed that the chromosomes of Rumex k-1 , R.tianschanicus L.ads and Rumex patientia L. were 2n-2=58,2n=20 and 2n=60. The size of the chromosomes of Rumex K-1 is small. Some are the dot chromosomes. The centromere is not distinct. It is necessary to study its multiply and base. 4. Rumex k-1's pollination habit is researched by castrating and covering with sack and manual pollination. The result showed that rumex k-1 can produce seed normally by either self pollination or cross pollination . The best time of cross pollination is 1-6 hour after blooming. The quantity of pollen is larger.The pollens are spreaded by wind, not by insect .Thereby , it should be paid more attention to isolation of seed production . 5. By observing the leaf horizontal microcosmic sections, the results showed that the leaf structure was consist of upside epidermis ,fence tissue ,sponge tissue ,downside epidermis . The vascular bundles were located among them. The costa had several vascular bundles and a great deal of thin wall tissue cells. It proved the theory of the water logging tolerence of Rumex K-1. 6. By way of paraffin , the condensed tannin-filled cells were not found in the leafy horizontal microcosmic sections of Rumex K-1 and the same was as in the lamina and the petiole with vanillan-HCl method. These results proved that there was not condensed tannins in the leafy section of Rumex K-1. On the otherhand, the condensed tannin was found in the calys and new seed capsule of Rumex k-1. Therefore the saying of Rumex k-1 having poor palatability because of the condensed tannin contained has no solid base. 7. As the resource of rumex k-1's chromosome is very complicated, so it should be paid more attention to build up the seed multiplying system and produce seeds. Otherwise, it will cause of the degrading of the variety. At meantime, it proved that the method for seed multiplication system taken by China Food Industry Group Romax Co.LTD is scientific and reasonable .
Keywords/Search Tags:Rumex K-1(Rumex patientia ×R.tianschanicus, cv.Rumex K-1) genetic stability chromosome pollination biology microscope observation Condensed tannin
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