Studies On Extraction Of Chenodeoxycholic Acid And Its Effects Of Immunity,Antitusis,Expectoration And Antiasthma

Chenodeoxycholic acid (CDCA) is one of active principles of chicken bile. Its content reaches 80 percent of bile acid from chicken bile. It was reported that CDCA inhibits cholesterol's synthesis in liver cells, makes ratio between cholesterol and cholic acid appropriate and has strong cholagogic action. CDCA can dissolve cholesterol calculus and be used to treat biliary calculi in clinic. There are three main parts in this paper. First, the CDCA has been extracted using biochemistry technology from Chicken bile. Second, experiments of immunity, antitusis, expectoration and antiasthma of CDCA have been carried out in animals. Last, by testing the content of second messenger-cAMP and NO in trachea tissue, the mechanism of actions above-mentioned has been discussed.In the research, CDCA has been extracted from Chicken bile by biochemistry technology of alkaline decomposition,acidification,extraction and confirmed its purity was reached 99.0% by using the silicate thin-layer chromatography and ultraviolet spectrophotometry. The recovery is 64.36% and the average content of CDCA in chicken bile is 23.2352g/l.The results show CDCA strengthens the nonspecific immunological function in high dose and low dose and inhibits significantly the specifc humoral immunological and cell immunological function of mice when it is given in high dose. But the result of delayed hypersensitivity induced by DNCB shows CDCA in low dose inhibits significantly the delayed hypersensitivity.The results of antitusis,expectoration and antiasthma experiments show CDCA can significantly control the cough of mice caused by NH3·H2O and SO2 and has strong resolving phlegm and antiasthmatic functions when it is given in 0.15g/kg and 0.225g/kg doses and by oral administration. Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. Then, NO content in serum and trachea was determinated with the method of spectroprotometry. The result of the test shows that CDCA can significantly reduce the quantity of NO in serum and trachea..