Studies On The Genetic Diversity Of Bombyx Mandarina M.and Expression Of The Toxin Specifically Acting On Insects

Studies on the genetic diversity of Bombyx mandarina M. play an important role in the facets of silkworms breeding, protection of wild resources, and construction of special properties genetic bases and so on. Nowadays, the most practical methods to analyze the genetic diversity are application of the technique of Random Amplified Polymorphic DNA (RAPD). It operates accurately, entirely and fast. Moreover its cost is low relatively too. So we applied the technique of RAPD for revealing the polymorphism of the Bombyx mandarina M. DNA comprehensively and roundly. The result showed that there were abundant diversities among the different origins wild silkworms. With the data gained from investigation of several origins Bombyx mandarina M. and the silkworm Su5, we had constructed the molecular phylogenetic tree of the Bombyx mandarina M.. In order to master the technology of gene engineering, we studied the Buthus martensii Karsch scorpion gene. It was expressed both in the E.coli and the insect cells, and detected with SDS-PAGE and western blot. The project, on the one hand, could pave the way to study the unpredictable gene detected by the RAPD and also could provide the theory warrant and the experimental materials to produce the gene engineering scorpion protein on the other hand.