Study On Technique System Of Tissue Culture In Litsea Lam

The stems of three breeds of Litsea cubeba,Litsea pungens and Litsea populifolid were used for explant to establish the technique system of tissue culture. To find out the best medium ,proceeding,breeding reaction of species of Litsea in vitro culture. And to find out effective measures to prevent the pollution and simplification methods. The results were as follows:Initiation medium:MS+BA2.0mg.r'+IBA0.5 mg.l-1. Mid November was the best season to get material. The first best species Litsea cubeba ,the result 4+4 minutes was good to treat the explant by HgCl2(0.1%).Subculture medium:MS+BA2.0 mg.r'+IBA0.01 mg.l-1 .The rate of propagation could reach about 7.7 for 30 days. After ten times ,the induction and differentiation came back normally,replacing MS by B5 and modified MS.Rooting medium:1/2MS+IBA0.1 mg.l-1+ AClg.l-1 Out bottle rooting,the lower part of stems was soaked in ABT 500 mg.l-1 solution for 8 seconds ,then cut in medium containing only sand.The procedure of simplified educates:at the permission range of the rate of pollution (5%).the boiled tap water replaced distilled water,the boiled bottle replaced the high pressure sterilization.