Research On Physiological Flower Bud Differentiation Mechanism Of Perrilla Frutescens(L.) Britte And Technology Of Extracting And Purifying α-Linolenic Acid

Physiological flower bud differentiation mechanism of Perrilla frutescens(L.) Britte and technology of extracting and purifying a -Linolenic acid were studied in this paper. 1 Physiological flower bud differentiation mechanismThe results showed that the phase of physiological flower bud differentiation could be divided into two phases: floral induction and floral initiation. The floral induction accomplished in the last ten-day period of June, and from which floral initiation began and accomplished in the last ten-day period of July.On this foundation, the author studied the effect of soil water stress and low-light stress on physiological flower bud differentiation of Perilla Frutescens(L.) Britte. The major results as follows:Copying DNA and transcribing RNA vigorously were essential for the physiological flower bud differentiation. The contents of DNA and RNA went up in the middle period of floral initiation, which obviously promoted physiological flower bud differentiation. The sharply reduce in value of RNA/DNA was a clear character for the physiological flower bud differentiation.Endogenous hormones regulated and controlled physiological flower bud differentiation by the way of adjusting, probably from time and space, the proportion or balance in themselves as well as nucleic acids metabolism.The major control as follows:Controlling by the time: peak value of endogenous hormones appeared in sequence: IAA and GA's (floral induction), ZRs's (early in floral initiation), ABA's(later in floral initiation).Controlling by the space: the change of IAA/GA's value probably dominated inthe floral induction. ZRs/GA's value possessed clear positive correlation with flower bud formation.The increase in carbohydrate and soluble protein were necessary in floral initiation, as might be related with synthesis of carbohydrate and enzyme needed in the formation of floral primordia. Total nitrogen content went on to descend after floral induction phase, and the lowest value of it came in middle period of floral bud initiation. C/N's value tended to rise between floral induction phase and early phase of floral initiation, and reached the lowest in the middle period of floral initiation. Relative high C/N's value probably was favor of accomplishment of the physiological flower bud differentiation. In addition, we also studied changes of the activity of a - AMY and NR as well as change of amylase isozyme band.The most evident effect of soil water stress on physiological flower bud differentiation was in the intermediary and later stage of floral initiation. The lasting soil water stress caused the harm of membrane system in this phase and restrained physiological flower bud differentiation. The most evident effect of low-light stress on physiological flower bud differentiation was in early stage of floral initiation. The lasting low-light stress obviously caused the speed of NAR lower than that of the control, and at the same time affected frequency of flower bud formation heavily.2 Technology of extracting and purifying a -Linolenic acidBased on studying the technology of extracting and purifying a -Linolenic acid, the results as follows:For extracting perilla seed oil, the best solvent was composed of petroleum ether and ethyl acetate. After perilla seed oil being extracted twice, each extraction lasting 12 hours at 60 癈, the average yield of perilla seed oil was 32.87%. Analyzed by gas chromatogram, the results showed that the content of a -Linolenic acid was 42.64%.In order to take-off the compositions such as a few dissociating fatty acids, phosphatides, pigments, proteins and so on., the perilla seed oil was refined by the treatment with NaOH solution. The results showed that the content of a -Linolenic acid, a -Linolenic acid yield and purification multiple were respectively 46.01%, 93.32% and 1.079 in refined perilla seed oil.On the base of comparing acid hydrolysis method and alkaline hydrolysis method, We improved the alkaline hydrolysis method.After being ref...