The Study On Porcine Myostatin Gene In 5' Promoter Region By PCR-RFLPs And Phylogenetic Relationships By RAPD

Pig is one of the most important economic animals for meat production nowadays, while lean meat percentage is one of the most important economic traits in pig breeding programs besides growing velocity, feed utilization ratio and litter size. Myostatin, which is a member of TGF-beta superfamily, was identified by Mcpherron (1997) as a negative regulator of skeletal muscle mass in mice and was relative to lean meat percentage. In this study we analyzed the polymorphisms of porcine myostatin gene in 5' promoter region among different breeds including Yorkshire, Landrace, Duroc, Junmu I and Min pig by PCR-RFLPs. The allele T dominates in the imported lean-type pig breeds such as Yorkshire, Landrace, Duroc. No allele A was detected in 40 individuals of Jumu I pigs, and the frequencies of these three genotype is about equal in Min pig. The result of X2 analysis showed that the distribution of three genotypes had very difference between Min pig and other breeds. Junmu I pig and other pigs also had very difference. At the same time, RAPD were used to study me genetic divergency and phylogenetic relation of these five breeds of domestic pigs. We selected fourteen primers from eighty random primers, caculated genetic distance index matrix and constructed phylogenetic tree using UPGMA methods. Genetic distance index matrix indicated that the genetic relationship between Junmu I pig and the Landrace was the closest, while that between Duroc and Min pig was the farthest.Postgraduate: Yang XiuqinSpeciality. Animal genetics and breedingAdvisor: Professor Liu Di...