Studies On Classification Of Pleurotus Nebrodensis With RAPD, RFLP And Physiology And Biochemistry Technique

Thirteen strains of Pleurotus nebrodensis and a strain of Pleurotus ostreatus collected from china were studied with the RAPD , isoenzyme technology,mycelial and fructification morphology.Identified at three levels of morphology,physiology & biochemistry and molecular biology,all strains of Pleurotus nebrodensis can be classified as different type.The mycelial morphology of Pleurotus nebrodensis in PDA plate are influnenced more greatly by invironmental condition than by their gentics. However, the fructification morphology of Pleurotus nebrodensis are opposed to mycelial morphology. In physiology and biochemistry methods, esterase isoenzyme technology can better classify Pleurotus nebrodensis than peroxidase isozyme and PPO isozyme technology. In molecular research, RAPD technology has higher delicacy than PCR-RFLP technology. A series of technology for the identification of strains with the RAPD technology and esterase isoenzyme technology, complemented with other physiology and biochemistry methods and morphology characteration were established in this tests. All the studies in this paper have the same results. This show that there are many ways to classify Pleurotus nebrodensis. Those strains of Pleurotus nebrodensis in China had accordant genetic type in high degree. So the cultivated strains of Pleurotus nebrodensis in China are few, and the naming of them is jumbled. Some strains with different name are the same one, but the other ones with the same name are the different strains in the market.