(GPV-SCa15) Of Gosling Plague And Its Characteristic

The results of studing on the growth of attenuated vaccine strain (GPV-SCal5) of Gosling Plague (GP) and its characteristic are report in this paper. An attenuated vaccine strain (GPV-SCal5) was obtained by passage in 10-day duck embryo for 15 passages. The strain' s characteristic as fellow: Deadly times was from 48h to 120h when inoculated GPV-SCal5 in 10-days duck embryo. Dead duck embryo chorion incrassate with grey putrescence dot, body hyperaemia, haemorrhage and edema, especially in head, neck, brisket and leg. ELD50 is 106.84/0.3ml (10-days duck embryo) , invirulence to one day old gosling, and not became virulence when growth in goslings and geese. The strain can reproduce in gosling's body after inoculated, and excreted into environment and infected goslings can get some immunity. Minimum immune dosage to goslings and geese is 1000 ELD50/0.3ml (10-days duck embryo) . 95 percent (19/20) goslings can against 100LD50/0.3ml (10-days goslings) virulence strain( GPV-SCv )3 days post inoculated (PI) and 100 percent (20/20)goslings can against lOOLD50/0.3ml (10-days goslings) GPV-SCv strain 5 days PI. 100 percent (20/20) goslings produced from parents that 15 days PI can against 100LD50/0.3ml (10-days goslings).The best immune effects to gosling are hypophloeodal injection and muscle injection, and orally, nose dropping and eyes dropping are next. The best immune effects to gosling are hypophloeodal injection and muscle injection, and nosedropping, eyes dropping and orally are next. Reproduce and virulence could be affected of GPV-SCv virulence strain by GPV~SCal5 strain when infect goslings in the same times.' Immunity time could be last over 30-days to gosling and about 5 months to geese. Immunity become lower to gosling when GPV-SCal5 strain pass 10-days duck embryo over 45 passages and to geese when GPV-SCal5 strain pass 10-days duck embryo over 42 passages. The immune effect could not be affected when GPV-SCal5 strain keep in -20℃ for 21 month, -4℃~0℃ for 13 month, 4℃ for 2 month and 20℃~25℃ for 5~8days.When GPV~SCal5 strain be tested in the 40 incubate factory, over 400 thousands gosling were inoculated and 70~90 percent are alive (the control goslings only 30~"50 percent alive). 90~95 percent goslings from vaccined parents are alive (the control goslings only 30~50 percent alive). GPV-SCal5 is stabilization, safety with excellent immunity.