Effect On Mechanism Of Gosling Plague Intestinal Injure And Recombinant Construction Of An Adenovirus Vectored Against VP3

Goose parvovirus (GPV) disease, a highly infectious and lethal disease in goslings, can causeserious loss in geese. The disease was noted among goslings and ducklings characterised by ascites,hydropericardium, hepatitis and myocarditis. The specific lesions observed in the affected geesepredominantly characterised by enteritis has also been described. Commonly used gosling plaguevaccine is basically attenuated vaccine attenuated vaccine gosling plague prevention but suchvaccines are still many shortcomings. In this study, we detected the expression of COX-2, iNOS,NF-kappaB, IL-1β and TNF-α mRNA using real-time quantitative PCR in intestinal damage. Forproviding the best products we constructed a recombinant replication-defection human adenovirusserotype5vector containing VP3protease coding regions, which can adenovius vector vaccine.Research as follow:1After gosling plague infection the mRNA expression of COX-2, iNOS, NF-kappaB, IL-1βand TNF-α significantly increased in goslings intestinal tissue, especially in the ileum and jejunum.GPV infection lead to upregulate the expression of inflammatory factors, caused the goslingsinflammatory bowel injury.2. Successfully constructed recombinant shuttle vector pShuttle-CMV-VP3. Homologousrecombination the adenovirus plasmid Pad-VP3, successful packaging of the adenovirus vaccinerAD-VP3in HEK293cells; recombinant viruses were identified by PCR and Weston-blot inHEK293cells.3. Detect recombinant adenovirus vaccine the immune efficacy trials in mice. Antibody titercan reach1:51200. We proved that the recombinant virus can induce mice to produce specificimmunity in serum by indirect ELISA.In summary, this study showed gosling plague suffering from myosoton organizationinflammatory lesions, COX-2, iNOS, NF-kappaB, IL-1β and TNF-α in inflammatory bowel injury.Build the new VP3gene adenovirus vaccine can generate a specific immune response in micegenetically engineered vaccine for the further development gosling plague some inspiration.