Study On Transformating Heat Shock Transcription Factor Gene Into Non-heading Chinese Cabbage (Brassica Campestris Ssp.chinensis Makino)

Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), a China originated leaf-vegetable, is very popular in China, particularly in South of China. However, seriously yield losses attacked by heat stress during summer season in this area. Many efforts on breeding to obtain heat-resistant non-heading Chinese cabbage had been done, but little of them made obviously progress. In this paper, transformation of HSF gene, a kind of heat-resistant gene, was employed and the risk of transformating HSF gene into non-heading Chinese cabbage was also discussed. Follow results were obtained.The regeneration frequency of Suzhou Qing was higher than that of Liang Baiye and AB line on media containing BA2mg/l, NAA0.45mg/l and AgNCMmg/l. The stable regeneration system based on the explants from cotyledons of Suzhou Qing was established.ABA had a significant effect on improving the regeneration frequency of Suzhou Qing cotyledons on the media containing AgNOs and ABA. GA had a stronger effect than ABA. The results showed that the adventitious shoots of non-heading Chinese cabbage were sensitive to ABA and GA.The regeneration plantlets were inhabited by antibiotic Kanamycin. The optimal concentration of Km was 10~15mg/l.The HSF gene was integrated into host genomic DNA of Suzhou Qing via Agribacterium tumefaciens and the PCR-Southern proved it. The RT-PCR results also showed that the HSF gene was already expressed.The injection of HSF gene via pollen-tube pathway was tried in this study. The PCR-Southern proved that the GUS gene and HSF gene were integrated into host genomic DNA. The results showed that the pollen-tube pathway was feasible to transformating foreign gene into non-heading Chinese cabbage.