| Tungseed meal is a kind of potential protein,abundant in china. It is of significance to exploit and make full use of it. In recent years,studies on Tungseed meal have been made great progress,especially in the method and technology of extraction and separation,evaluation of nutrition value of Tungseed meal and its application to livestock. Up till now,toxin in Tungseed meal hasn' t been made clear,and no approach to evaluation of toxin in Tungseed meal has been reported,which hindered the use of Tungseed meal in livestock production . Based on previous researches,a method of determination of Ester-soluble crude toxin in Tungseed meal is developed in the present study .The detoxicatibn effects were compared among different treatments with the developed method,the remaining levels of toxin in detoxicated Tungseed meal were determined and evaluation of safety of detoxicated Tungseed meal is investigated.The results of this study reveals that Ester-soluble crude toxin meal extracted by ethanol solution and ETOAC ( ethyl acetate ) is of ultraviolet absorbance and the spectrogram of Ester-soluble crude toxin has the peak absorption at 270nm,and there is a positive correlation between concentration(Y) and absorbance (X).Y=28. 109X+2. 4533,R2=0. 9993. When the ultraviolet absorbance of Ester-soluble crude toxin solution is determined by UV-spectrophometry,the weight of Ester-solution crude toxion in Tungseed meal can be estimated according to regression formula and total volume of Ester-soluble crude toxin solution . The estimation equation is M=4(28. 109A+2. 4533) X 10-2(g) . A comparison with the true values shows that the measured values of four different Ester-soluble crude toxin level mixed samples by this mean is close,and the bia between true values and the measured values is less than 5%,which suggested that this method may be applicable to the determinationof the Ester-soluble crude toxin and accurate as well.The application of the developed method to the investigation on the effects of detoxication among different treatments showed that different treatments resulted in different detoxication effects . The longer detoxication lasted,the better the effect was. The effect of detoxication improved by 10% when detoxication prolonged 48 hours. The detoxin solvent concentration also affected the effects of detoxication. The higher the concentration of ethanol solvent,the better the effects of detoxication . The effects of 75% of ethanol solution was about two times better than that of 50% of ethanol and the effects of 95% of ethanol was about three times better than that of 50% of ethanol. The detoxication effects were affected by different temperature. The higher the temperature was,the better the effect of detoxication. .However,the effect of detoxication was increased slowly when the temperature was increased rapidly. Natural fermentation also affected the effects of detoxication . The concentration of Ester-soluble crude toxin in Tungseed meal was decreased by 10% when it was fermented for 24 hours at indoor temperature 32 C humidity 40%.The content of crude toxin extracted from three kinds of Tungseed meal,which were respectively obtained from the oil plants of Cili County,Yongshun County and Hongjiang County of Hunan Province,was determined in this way. The result showed that there was some difference among those content. The content of crude toxin in Tungseed meal from the oil plant of Yongshun was the lowest,while that of Hongjiang wasthe highest.The evaluation of the safety of detoxified Tungseed meal was preliminarily investigated with the developed method,the method is also applied to the test of the detoxincated Tungseed meal when the safety of the detoxified Tungseed meal was proved by the feeding experiment and acute toxin experiment of mice,The results suggested that the detoxified Tungseed meal was safe when the absorbance of detoxified solution of ester-soluble crude toxin extracted by ethanol and ethyl acetate at 270nm is less than 0. 329 and the weightof Ester-soluble crude to...
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