| The common edelweiss (Leontopodium leontopodioides),as a folk herb,is the whole herb of leontopodioides of Leontopodium plants of Compositae. The folk clinical researchs indicated that the herb possesses the effects of dispersing the pathogenic wind and expelling superficial evils,clearing away heat and toxic materials,cooling the blood and stopping bleeding,invigorating the kidney and promoting water,anti-inflammatory and promoting diuresis,and so on. Literature retrieval and field investigation showed that the natural resources of this plant are rich very much;the research reports concerned are rare in china;the reports on the chemistry components and pharmacological activities of this plant are not found in abroad yet. Obviously,its active components and their pharmacological activities need farther studied for exploitage application of the common edelweiss. Mastitis is a kind of inflammation of bovine mammary gland,which is a complicated disease that brought the most seriously economic losses to the bovine industry in the whole world. Although some western countries have studied this disease for over one hundred years,a thorough solvation is not put forward up to today. In recent more twenty years,even though our country has made marked achievement in the research of mastitis,there is a very long way to go before this problem is solved completely. In the prevention and treatment of bovine mastitis,traditional antibiotic therapy brought a series of questions,such as resistant organism,antibiotic residual,and so on. Therefore,non-antibiotic therapy has become a research focus in domestic and abroad. Chinese medicinal herb and its active constituents were used to prevent and treat mastits,as a important part of non-antibiotic therapy,is becoming increasingly the centre of attention of the specialists in mastitis study in the world.The antibacterial,anti-inflammatory and immune activity of the different polar fractions of the common edelweiss were studied for the prevention and treatment of mastitis. This research laid a foundation for the development and application of the herb. 1 Collection of Leontopodium leontopodioides,identification of crude drug,extraction and isolation of active constituents.The Latin name of the common edelweiss from Gingzang Plateau was determined by thetaxonnmy identification method. The water decoction,the method of water-extraction and alcohol precipitation,and the alcohol refluxing method were used to extract the active principles of this whole herb. By using the extract method,the alcohol extraction was isolated into four different polar fractions,such as the petroleum ether-soluble fraction,the ethylacetate-soluble fraction,the n-butanol-soluble fraction and the water-soluble fraction. In the pharmacological trials,seven different polar fractions as experimental drugs are respectively the decoction,the alcohol extraction and its the petroleum ether-soluble fraction,the ethylacetate-soluble fraction,the n-butanol-soluble fraction and the water-soluble fraction.2 Study on the antibacterial activity in vitro of Leontopodium leontopodioides.The test tube dilution method was adopted to measure minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of seven extractions (such as the decoction,the solution of water-extraction and alcohol precipitation,the alcohol extraction and its the petroleum ether-soluble fraction,the ethylacetate-soluble fraction,the n-butanol-soluble fraction and the water-soluble fraction) against six bacteria,such as E.coli Cg3882,E.coli Cg3903,E.coli Cg39i4,Salmonella €79-20,Staphylococcus aureus Newbould S-305,and Staphylococcus aureus isolated from the clinical case of bovine mastitis. The experimental results indicated that the alcohol extraction and its the petroleum ether-soluble fraction and the ethylacetate-soluble fraction can strongly inhibit the development of Staphylococcus aureus with MIC(0.14mglml) and MBC(0.27mg/ml). The n-butanol-soluble fraction and the water-soluble fraction of the alcohol extra...
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