Study On Molecular Polymorphisms Of CAST And HFABPL Gene In Beef And Their Relationships With Meat Quality Traits

The purpose of the study is to detect the genetic variations of CAST gene and HFABPL gene by PCR-RFLP, after the polymorphism fragments were cloned and sequenced we can identify the corresponding restriction sites and the mutative base. Analysis of variance was conducted to investigate effects of the genotypes on meat tenderness and Marbling traits in 176 cattle.A total of 176 cattle from 7 breeds (Angus x Luxi, Simmental x Luxi, Charolais x, Luxi Limousin x Luxi ,purebred Luxi, purebred Jinnnan, purebred Qinchuan) were examined. The amplified fragment of bovine CAST gene intron6 and HFABP exons were estimated to be approximately 1685 and 613bp respectively. Several restriction enzymes were used to digest the PCR product and the Xmn I and Rsa I -RFLP were confirmed. Two allelic fragments were produced and genotypes were assigned as BB for which has the restriction site in CAST and AA in HFABP. A PGEM T easy cloning vector was used for PCR doing and sequencing was conducted at SHEN YOU company. The sequence revealed a single nucleotide sustitution of C-G at position 1015 for Xmn I site and a G-A at position 298 for Rsa I. The frequencies of CAST gene B allele and HFABPL gene A allele in 7 breed was about 0.57 and 0.78. CAST BB genotype significantly influenced tenderness(p<0.05) but no in Marbling, and in Limousin crossbred it has highly significant positive effect on WBS(P<0.01). HFABP genotypes does not have any effect on Marbling and tenderness traits.