Analysis On Sequence And Structure And Genetics Diversity By Domestic Geese MtDNA

The mtDNA of altogether 25 individuals in 6 breeds of Chinese goose and 2 breeds of domestic Europe goose were extractioned and the entire cytochrome b (Cyt b) gene (1143bp), ND5 gene partial sequences (77bp) along with 2tRNA gene (137bp) were sequenced. Comparability of these sequences and the cloverleaf structure of 2tRNA gene and the transmembrane helices structure of the entire Cyt b gene and variation of sequence were analysed hi the paper. The results indicated as follow:The analogical percentage between determined sequences of gooseand white-front goose (Anser albifrons), dabbling duck(Aythya Americana) inGenbank was 97%, 88% respectively. The analogical percentage between domestic goose and white-front goose in Genbank about ammo acid list of Cyt b was more than 99%, The reliability of the sequence was testified by comparability and the cloverleaf structure of 2tRNA and the transmembrane helices structure of the entire Cyt b gene between domestic geese and white-front goose; The amino acid arm and anticodon loop of cloverleaf structure of 2tRNA is very conservative among Anser albifrons and Anser cmser and Anser cygnoides. The sequence in the intermembrane domain of Cyt b transmemb helices was relatively conservative among Anser albifrons and Anse anser eand Anser cygnoides;variable sites in matrix domain, in the intermembrane domain and outer membrane was 12, 9 and 10 respectively, variation both in matrix domain and in the intermembrane domain were synonymious substitution, two various site were nonsynonymious substitution outer membrane, the difference outer membrane was more severity than matrix domain and in the intermembrane. 22 variable sites and 3 haplotypes was detected in 25 sequences, nucleotide diversity (Pi), haplotype diversity (H) and average number of nucleotide differences(K) were estimated to be 0.00775,0.547,10.86 respectively; There are two origins in domestic geese breeds, namely Xingjiang Yili breed and Landoise breed along with Rhin breed originated from Anser anser and other 5 Chinese goose breeds originated from Anser cygnoides; The genetic differentiation interspecific of goose could be examined by the molecule marker with the partial sequence of mtDNA including the entire cytochrome b (Cyt b) gene (1143bp), ND5 gene partial list (77bp) along with 2tRNA gene (137bp),whereas the genetic differentiation among geese breeds could not be checked up by the molecule marker.