Study On Genetic Diversity Of Native Goose Breeds In Zhejiang Province

To study the genetic diversity and relationship of native geese in Zhejiang Province, the complete mitochondrial control regions of five local and one European domestic goose breeds (96 individuals in total) were sequenced. The result showed that the lengths of the complete D-loop sequence were 1166-1179bp. Eighty-nine variable sites and four mutation types (transition, transversion, insertion and deletion) were detected, and the frequency of conversion was much higher than other mutation types. In this study the haplotype diversity was 0.00862 and 36 haplotypes were identified, H3 was the major shared haplotype among 6 breeds.There were some differences among different domestic goose breeds in nucleotide diversity. The nucleotide diversity of Panshi Grey Goose (0.01641) was the highest, higher than that of Zhedong White Goose (0.00897), Yongkang Grey Goose (0.00586), Jiangshan White Goose (0.00673), Taihu Goose (0.00418), Landoise Goose (0.00092). In addition, Panshi Grey Goose had the most haplotype and high haplotype diversity, so the Panshi Grey Goose showed the most genetic diversity and the Landoise Goose the least.The genetic distance of Panshi Grey Goose (0.01043～0.01293) was significantly higher than that of other goose breeds (0.00255～0.00768). The genetic distance between Panshi Grey Goose and Yongkang Grey Goose was 0.01211, much higher than the nonrmal genetic distance between domestic goose breeds, indicating that the two groups were not the same goose breed.The phylogenetic analysis was based on the complete sequences of the mitochondrial control region by Minimum Evolution Method, Maximum Parsimony Method, Neighbor-Joining Method and Unweighted Pair-Group Method with Arithemtic Mean Method and 4 trees were obtained. The result showed that the species in the same genera were well clusterd together respectively, however the goose breeds in Anser anser and Anser cygnoides were not clustered together, indicating their maternal origins were random and multiple, and the breed selection was mainly focused on paternal side, which requires a further combined analysis of goose genetic background from genomic level.