The Biological Characteristics And RAPD Analysis Of Quiescent Colletotrichum Musae Resistant To Carbendazin In Banana Fruit

29 single spore strains of Colletotrichum musae were seperated from 9 different level of TBZ-resistant isolates and 1 TBZ-sensitive isolate.Level of resistance to carbendazin and biological characteristics of all strains were tested.Random amplified polymorphic DNA (RAPD) was used to distinguish between carbendazin -sensitivity and resistance strains.The results showed that ECso values of 29 strains were significantly different,varying from 0.04 u g/ml to 686.57 u g/ml.Sensitive and R/S < 6 strains were similar in colonymorphology, growth of mycelium,pathogenicity and capability of sporulation,showing great difference to those of R/S>6 stains. After different temperature.PH value.carbon source and illumination treatments , growth of mycelium in sensitive and R/S < 6 stains was faster than that of R/S >6 stains.There was irregular variation in spore germination between sensitive and resistant strains.After 8 succesive transfers and incubation on the fungicide free PDA, EC50 values of all strains did not change notablly.suggesting that the resistance of these strains was stable. 6 out of 40 random primers (10-mer) could stablly amplified the genomic DNA from the tested strains. 71 RAPD bands were obtained ,70.42% were polymorphisms. A dendrogrem was constructed using UPGMA software for clustering analysis.lt indicated that at 0.42 genetic distance ,29 strains could be classified into two groups.One included sensitive and R/S < 6 stains.the other included R/S ^ 6 stains.The classification was obviously related to their biological characteristics and fungicide resistance.