Apple-pear Variant Monophyletic Biological Characteristics Of Survey And Rapd Polymorphism Analysis

Applying Pingguoli(Pyrus ussuriensis var.ovoidea_XP.Pyrifolia)and its eleven varied strains as testing materials,several good strains of synthetical properties were screened out of the others through the investigation of biological characteristics and the analysis of fruits quality. On base of these, using the ripe and fresh leaves of Pingguoli and its eleven variation strains, genome DNA was abstracted and polymorphic of RAPD was studied. The results showed that:Some genetic variation occurred in the course of spreeding and cultivating of Pingguoli .In all varied strains, there were some divergences in quality of fruit, and the exceptional or synthetical characters of some varied strains were better than Pingguoli .By identifying and analyzing, four varied strains whose synthetical character were better were P₁,P₅,P₇,P₈.An improved CTAB method could extracted high-quality DNA from gene group of Pingguoli and its variation strains and filter eighteen 10 random primer in RAPD amplification procedures, their polymorphism rate were over 67.7. An optimal reaction systern for RAPD-PCR was: template DNA 15ng/u L, Mg²⁺ 1.0mmol/L, Taq DNA polymerase 1 unit, dNTP 1.0mmol/L, 10-code primer 15pmol, 10×buffer 2.5mmol/L, others complement with ddH₂O. The amplification procedures was: initial denaturation at 94^C for 5 minutes; denaturation at 94^C for 1 minute, annealing at 37 for 1 minute, elongation at 72^C for 1.5minutes, 45 cycles; final elongation at 72^C for 7 minutes, stop at 4^C.Fingerprints of Pingguoli and its eleven varied strains were established .The marker of P₂å'ŒP₇ were founded, 11 variation strains can be distinguished from from Pingguoli in one primer.