Study On In Vitro Regeneration And Somaclonal Variation Of Cyclamen Persicum Mill

In vitro regeneration of Cyclamen 'dense fragrance' has been attempted in this experiment. Two kinds of auxins were applied respectively to induce the callus. They were 2,4-D and NAA. Then separate experiments and rooting experiments were carried out. The aspect of successive transfer culture was involved too. In the experiment, The question of browning of explants were studied. In order to get variant by chmomorphosis, callus were induced to produce variant by mutagen colchicum. And the variants were detected by methods of morphology observing and electrophoresis of isoenzyme. The results showed:It was found that the callus from young leaves was easy to induce and grew rapidly. The best inducing medium is MS add 2,4-D0.5mg/L, BA0.2mg/L, the differentiation medium is MS add BA2mg/L, 2,4-D 0.5mg/L and KT 0.2mg/L. Then the explants should be transfer into 1/2MS add IBA0.2mg/L and BA0.02mg/L to root. The differentiation medium were also suitable for the callus induced by NAA. the suitable media for subculture respectively were MS add 2,4-D0.5mg/L and MS add NAA 1mg/L towards the callus induced by 2,4-D or NAA. For adventitious bud multiplication, the caltures with shoot buds should be transfered into MS added BAlmg/L, 2,4-D 0.5mg/L and KT 0.2mg/L.Appreciate anti-browning agents and concentration were Vc200mg/L, PVP0.04%.Time and concentration of treatment of colchicum affect mortality and frequency of differentiation of callus. The proper combination of time and concentration of colchicum treatment was 100mg/L and 15 day.Either the regenerative plant induced by 2,4-D or that of by NAA, The rate of somaclonal variation was great in Cyclamen in vitro regeneration. After the treatment of colchicum, the rate of variation increased. These plantlets often showed deviant phenotypes.