Cloning, Sequence Analysis And Detection By In Situ Hybridization Of Jaagsiekte Retrovirus ENV Gene

Sheep pulmonary adenomatosis (SPA) is a contagious and experimentally transmissible lung cancer of sheep resembling human bronchiolo-alveolar carcinoma,SPA is a nature animal model in study of BAC.A type D retrovirus,known as jaagiekte sheep retrovirus(JSRV),has been associated with the etiology of, SPA.Four case sheep lung tissues were subjected to pathological histopothologic and PCR examination.The results show that examinated sheep were affected by JSRV.For the first time,the complete long env gene of the Jaagsiekte sheep retrovirus (JSRV) has been colpned from Provimses in China in sheep genome and sequeneed,the gene is 1836 nucleotides long .There are 12 nucleotides mutations compared with type I and type II JSRV counterparts the sequences of the virus was 87% to 88% identical to the described three JSRV env sequences.The env protein deduced from env gene encodes the hydrophilic surface protein(SU) and the hydrophobic transmembrane domain(TM) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry.The SU of retroviruses is a highly variable genetic element,containing receptor binding sites and major antigenic determinants.exJSRV-specific DNA probes were derived.By using these DNA probes in tissue hybridization. We successfully identified JSRV mRNA expression and proviruses DNA in sheep lung tissues infected with JSRV and control group has no postive signals,Validating the use of exogenous virus-specific DNA probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences.