Study On Genetic Diversity Of Four Trematodes In Opisthorchiidae

4 species of trematodes in Opishorchiidae(Metorchis taiwanensis, Amphimerus anatis, Clonorchis sinensis^ Microtrema truncation) were used for genetic diversity analysis using random amplified polymorphic DNA technique(RAPD). The result indicated that template DNA , which extracted by improved phenol/ chloroform method proved to be high quality and high purity. The ampilification reaction system and PCR programe were proved suitable through filtrating and optimizing repeatedly. The result indicated that 20 primers were screened from 40 random 10-mer primers, and a total of 310 DNA bands were amplified, 307 of which (about 99.03%) were polymophic bands. The number of amplified bands by each primer ranged from 6 to 23 with size from 202bp to 2460bp. Primer S22 amplified the greatest number of bands (23 bands), primer S477 amplified the least number of bands (6 bands). Primer S130, S232 and S341 amplified common bands of 4 speices trematode. The dendrogram derived from RAPD data by AVERAGE suggested that genetic distance between Amphimerus anatis and Metorchis taiwanensi was 0.7944 they clustered together first. Genetic distance between Microtrema truncatum and Metorchis taiwanensis was furthest (0.9306), they clustered together last. The result was in agree with the point of traditional classification. RAPD could be used in detection of genetic diversity and analysis of genetic relationship of 4 species of trematodes in Opishorchiidae.