| Molecular breeding is to breed new plant variety via improving or modifying genetic character of crop. The cowpea trypsin inhibitor (CpTI) gene and the bar gene discovered in recent years are found to be used for insect-resistant and anti -herbicide respectively because of their broad spectrum and are used in gene improvement of many crops widely now. Using the peanut (Arachis hypogaea L. cv. Shanyou 523 and Arachis hypogaea L. cv. Shanyou 21) in this report, method of transforming the CpTI and bar gene to peanut with pollen tube pathway technique was studied, the mechanism of the technique in the peanut was discussed; biochemic ingredient changing among the peanut cultivars was analyzed, and genetic distance was studied with RAPD analysis, we hope that these study will be useful to crop breeding.In the two analyses different 19 peanut cultivars were analyzed by RAPD technique respectively. The result shows there are plenty of polymorphism among the peanut cultivars. In the first analysis, rate of polymorphic fragments is 90.5%, 19 peanut cultivars is classified in 5 categories, the parent and filial generation cultivars is classified in the same categories approximately; In the second analysis, rate of polymorphic fragments is 83.5%, 19 peanut cultivars is classified in 5 categories approximately, the series of cv. Shanyou are classified in a category, the parent and filial generation cultivars is classified in the same category approximately too. The two analyses show the UPGMA tree is accord with the relationship of the cultivars. The differences among the peanut cultivars are small. The mean genetic distance of the two analyses is 0.313 and 0.261 respectively. It may provide the identification of the peanut cultivars or transgenic plant with proof via RAPD polymorphism. In this report we identified three questionable peanut cultivars via RAPD analysis, the result shows that the cv.Yuexuan58/l is parent of the cv.Wufeisi.But in two analyses, there is 4 repeat cultivars. In the two RAPD distancematrix, between the same pairs the difference of distance is significant, for example, the distance between the pair of the cv.Zhanyou 30 and cv.NCAC 17090 is 0.3617 and 0.2561 respectively, between cv.Shanyou 523 and cv. Shitouqi is 0.2319 and 0.1847 respectively; in the two RAPD trees ,the four cultivars lies in different categories. It shows that repeat of the RAPD analysis is poor.Biochemic ingredient data and morphological data of the cv.Zhanyou 30 and its parents cv.Shanyou 523 and cv.Yueyou 223 was analyzed, the mean value difference was tested by Ttest. The result shows that protein, raw fat and morphological data between the cv.Zhanyou 30 and its one of two parents always is different significantly except the fatty acid data. These indicate that biochemic ingredient and morphological character can be inherited steadily via crossbreeding.Comparing the result of the first RAPD analysis with the result of the biochemic analysis ,the difference of the pair(cv.Jinhual012 and cv.Tianjindou) which distance is longest is significant in the protein ,raw fat and the main fatty acid ,but only in the raw fat the difference between the cv.Jinhua!012 and cv.Yueyou 256(distance between the pair is very close) is not significant; In the second RAPD analysis, the difference of the pair(cv.Shanyou27 and cv. NCAC 17090)which distance is longest is not significant in the protein and oleic acid, only in the raw fat the difference between the cv.Shanyou27 and ev.Shiyou!5 (distance between the pair is very close) is not significant .From all above ,we thought that the long distance is not consonant with the biochemic ingredient difference.In order to increase the translation efficiency of the pollen tube pathway technique, comparing the shanyou523 with shanyou21 in the blossom rule. The result shows that the two cultivars' full-blossom stage is about 8-10 days respectively, in this stage every peanut blossom out more than 3 flowers. In the spring, the flower number per peanut plant is fluctuant widely; but in the...
|
PDF Full Text Request