| The wide application and rapid development of genetic engineering open up a broader path for soybean genetic improvement. In this study, transformation of soybean(N2899) with gagal was conducted succesfully, through pollen pathway in 2000.On this basis, the effect of gagal expression to the growth and development , agronomic traits and seed protein composition of soybean was studied .The main results are as follows:1) No obvious modification was found in floral structure compared to untransformed N2899 in T1-T3 transgenic soybeans.2) For the agronomic traits,it is found that Tl transgenic plants were shorter and had less flowers during full blooming period; T3 transgenic plants was shorter and had lighter 100-seed weight than CK. That is to say, T3 transgenic plants were 10cm shorter than CK at seedling stage, 27cm shorter and 7g lighter than CK at mature stage.3) For the seed storage protein component and content, it was found that T3 transgenic soybeans were divided into three groups.The first group included Nol and No4 communities , in which both 7S and 11S globulins content were lower than CK. The content of two polypeptide between 25KD-3 1KD was obviously higher than CK. Hence, the 11 S/7S ratio was higher than CK.The second group included No2 , No3 and No5 communities , in which 7S globulin content was higher than CK, IIS globulin content and 11S/7S ratio were lower than CK.The third group included No6 , No7 and No8 communities , in which 7S and 1 IS globulin content and 11S/7S ratio were lower than CK.This phenomenon was probably caused by insertion of multi-copies ofgenes.lt is the first evidence that gagal is involved in the regulation of seed protein composition in soybean.4) In addition, gagal ,g2psl and geg were transfered by pollen tube pathway into soybean in 2002, 9, 22 , 3 plants with resistance to Km were found respectively. Meanwhile ,soybean was transformed with gaga2 by seed-soaked way in 2002, 6 plants with resistance to Km were identified. |
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