Mass Production Of Prunus Serrulats Tube-shoots And Some Problems And Countermeasures On Forest Trees Micropropagation In Factory

Shoot tissue culture now be consided of one of the best methods in micropropagation. which can assure plant genetic types. That's why some vegetables produced in industry by this way, and it is also more economic than other methods.We builded shoot tissue culture systems of four kinds of P. serrulata. Results obtained from these studies show that, it's the best time for getting new budes of P. serrulata from March to April. The medium Jl( 1/2MS + 6BA0.50 + NAA0.025) is suitable for explant induction, initial culture and subculture, but the explants obtained after May can be cultured in the medium C4(1/2MS+6BA1.0+NAA0.02). The tube-shoots will appear rosellar phenomina after two subcultures, which can effect the growth of tube-shoots seriously, but the optimal medium J11 (1/2MS +6BA0.50 + NAA0.025+GA0.025) can promote elongation of the axillery buds, so the rosella will disappear after one subculture, but GA can not be used too long or in high concentration, because of its resulting in thin stem and excessive growth of tube-shoots. There is another problem, the tube-shoots in J1 and J11 mediums are too tender to be rooted, but the medium J14 (1/2MS +6BA(0.5mg/L)) can resolve the problem better. After several subcultures, the tube-shoots grow slowly little by little. Some investigations discoved that the disorder or higher concentration of hormenes or regulations in plants rleading to the phenomena. We found that the tube-shoots recovered growth after being cultured in the medium J18 (1/2MS) about 20 days. We also found that the growth of four kinds of P. serrulata have no- significant discrepancies in those mediums. That is to say, It's valuable for mass production of many kinds of P. serrulata by simplifmg the mediums and solving some problems in the course.There are some problems affecting the application of forest trees tissue culture inindustry. Vitrification is around in plant tissue culture, we concluded that suger, agar, light, pH, ionic concentration and contamination proof closure modes effected tube-shoot vitrification from the experiments, suger and agar can inhibit vitrification when their concentration raised to some extent, that will also be happen when air circulation between inner and outer of culture vessels become better and higher ionic concentration and light intensity. On the other side, vitrification are different among four kinds of P. serrulata.GA promotes tube-shoots elongation, but using it longer(about several subcultures) can lead to attenuation of the tube-shoots, such as stem slightness, leaf etiolation, reducing tube-shoots survive rate, and so on.. PP333 can restrain those affections by GA, and it will promote Robinia axillery buds high-frequently occuring if the medium added PP333 (0.05mg/L).A. Palmatum and R. dauricum were used in the studies on the optimal explants. We consider that the best time of collecting explants is spring and it is the seem with others'opinions. On the other side, it is not aways right geting too young explants, especially in forest trees tissue culture. But the half-lignification explants is better than orthers.