| The yield of oil rape (Cruciferae Barssica ) is badly affected and damaged by insect cutile. The widely use of chemical pesticides inevitably brings about serious safety and pollution problems. With the development of biotechnology, the pest resistant transgenic plants by introducing exterior anti-pest genes into the crops is a new way.In this project, the petioles from the sterile seedlings of Brassica juncea (33B, 918B) were used as experimental materials. The explants were infected and cocultured with Agrobacterium tumfaciens EH A105 containing pB1101 which carries scorpion toxin and chitinase genes.Factors influencing B. juncea transformation mediated by A.tumfaciens have been studied The results showed that sterilization of oil rape seeds using 0.1% HgCl2 was better for the growth of sterile seedling. The petioles from 5d old sterile seedlings had the highest differentiation frequency and were appropriate for transformation. When the petioles were infected by A.tumfaciens for 10 min, the efficiency of transformation was the highest and the callus had the lest browning. Considering antibiotics' bacteriostatic activity and differentiation of petioles, 500mg/L carbenicillin was selected for excluding A tumefaciens and 10mg/L kanamycin was for screening explants. An efficient and stable B. juncea transgenic system was established.The binary anti-insect genes were transferred into B. juncea via A.tumfaciens-medlated transformation. 153 kanamycin-resistant shoots were produced during 36 patens of experiment. The regeneration ratio was about 2%. After transferring them into flower-pots 21 regenerated plants survived. The PCR result showed that the insect-resistant genes had been integrated into the genome of taransgenic plants. All the transformants were normal in morphology.
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