Etiological Detection Of Human Viral Hepatitis And Pathological Study On The Liver Of The Slautered Swine

We designed these experiments in order to know the meet hygiene situation and detect Viral Hepatitis in the slaughtered swine. These results will be helpful to the administration of swine slaughter, epidemiology and vaccine study of the viral hepatitis. We hope that the reseacher should advert to this problem.We desinged 7 experiments to provide enough evidence.1 Serology detection in slaughtered swine.2 Histilogy and Pathology study in slaughtered swine.3 HBcAg and HBsAg immunohistochemical detection in slaughtered swine.4 Apoptosis detection in slaughtered swine.5 Electron Microscope dection in slaughtered swine.6 HBV DNA PCR detection in slaughtered swine.7 HCV immunohistochemical detection in slaughtered swine. Result:There are total 136 liver samples of slaughtered swine. It was found that 48 cases of all detected samples (35%) were positive for both the HBsAg and HBcAg. 25 cases (18%) appeared positive HBcAg, but negative HBsAg. In these HBV antigen (HBsAg, HBcAg) positive samples, we use anti-Bcl-2/Bax and TUNEL method to detect apoptosis in these liver samples. There are 8 samples anti-Bax positive with Bcl-2 negative, 3 samples anti-Bcl-2 positive with Bax negative. The TUNEL result is the same as anti-Bax result, 8 samples corresponding positive. PCR result shows no positive samples. In these livers some virus particles are also founded under EM. There are 5 samples positive, 8% of total.These results indicate that there is Hepatitis virus especially HBV in these slaughtered swine's livers, but not the same as the Human HBV. We suggest that the public health department and hepatitis researcher must pay attention to this problem.