RAPD Markers And Their Genetic Diversity Among Three Species Of Monopterus

The samples of three species of synbranchi form eels, Monopterus cuchia (Hamilton, 1822), M fossorius (Nayar, 1952) and M. albus (Zuiew,793) were collected from Yangon of Myanmar, Indonesia and YuanJiang County, Hunan Province,China respectively. Randomly amplified polymorphic DNA (RAPD) technique is usded to analyse the specific genetic markers and their genefic diversity of the wild grouper population of four individuals from each species.The reactions were performed through 94 #, pre-denaturing for 5 min, then 45 cycles of 94 # denaturing for 1 min, 36# annealing for 1 min and 72# polymerizing for 2 min; The complete extention was conducted at 72 # for 7 min. 40 primers of OPA, OPB and OPD were screened, 30 primers produced reproducible fingerprints with the molecular weight varying from 250 bp to 5417 bp. The number of RAPD markers amplified by each primer varied from 2 to 16. Those interpretable patterns generated by 30 primers were used as the genetic markers to discriminate the differentiation of the three species. 87 out of 315 amplified fragments were distinctive for the three species, 33 were from M. fossorius, 25 and 29 were from M. cuchia and M. albus respectively. The percentage of polymorphic ioci(P) among inter population of three species is 80.32%. The genetic diversity index (H) among inter population of three species is 0.157 5. Based on the RAPD data, the genetic distances among these three species of Monopterus, were computed by using DPS2003 software package. As result, the biggest genetic distance occurres between M. fossorius and M.albu with a value of 0.7415, while the smallest genetic distance with a value of 0.6860 comes about between M. cuchia and M. albus, and the genetic distance between M. cuchia and M. fossorius is 0.7035. The cluster analysis of the above three species was performed with UPGMA algorithm over their genetic distances. The dendrogram of these three species was constructed.