Studies On The Basic Biology Of TGMS Rice With Eui Gene

Changxuan 3S was thermo-sensitive male sterile(TGMS) rice with eui gene selected from seeds of Peiai 64S irradiated by 350 Gy60Co Y -ray. To provide science evidence and theory support for exploiting and utilizing TGMS rice Changxuan 3S with eui gene, the correlativity between panicle exsertion of Changxuan 3S and nature temperature was analyzed, and effect of different temperature treatment on eui gene expression was researched, and the cell number and length were analyzed. The main results were as follows:(1) There was a negative correlativity of panicle exsertion and nature temperature at 9-20 days before flowering. And the negative correlativity of panicle exsertion with mean diurnal temperature at 12-17 days(from pollen mother cell formation to meiosis) which was the thermo-sensitive period of eui gene was the most significant before flowering. The analysis showed that eui gene expressed best in feasible temperature for 6 days during the thermo-sensitive period.(2) In the thermo-sensitive period of eui gene, when Changxuan 3S was treated with the condition of constant 22C, 24 C, 26C and 28C respectively, the eui gene could express under the treatment of 22C, 24C and 26C respectively but was inhibited at temperature 28C. And the lower the temperature treated, the longer the panicle exsertion was.(3) The dynamic mutative rule of uppermost internode elongation compared with Peiai 64S was concluded from the statistic of first internode length in different stage. The rule was that the uppermost internode of Changxuan 3S elongated much more from VII stage to flowering day, but had little difference with Peiai 64S before VII stage. The observation of first internode cell length in different stages also made the similarrule which the cell elongated faster from VII stage.(4) The spatial mutative rule of uppermost internode cell elongation of thermo-sensitive rice with eui gene was researched from the cell number statistic and length measure. The result of cell comparison showed that uppermost internode cell length in different temperature treatment was different which the cell length of first internode with the treatment of 22 C and 24 C was longer than that in 26C and 28C. Uppermost internode of Changxuan 3S was 18. 0 cm longer than that of Peiai 64S. And the number of external and internal thin-walled cell were 1 248 and 580 more than those of Peiai 64S respectively, length of these two kinds cell of Changxuan 3S were 23. 3um and 38. 3 u m more than those of Peiai 64S respectively. The length of external and internal thin-walled cell of Changxuan 3S in the middle section of uppermost internode was 24. 8um and 48. 7um longer than those of Peiai 64S extremely. Cell length of Changxuan 3S increased abruptly from group section to middle section, and internal thin-walled cell elongated more extremely. It is, therefore, evident that the uppermost internode elongation of Changxuan 3S is due to the increase of cell numbers and cell elongation, and the effect of later was more obviously.(5) It was found that there were tow kinds of band pattern in mRNA of Changxuan 3S with the treatment of 24C. One kind was the special band and another was the type of strong band.(6) The fertility of Changxuan 3S had a little change after the temperature treatment of 22C during the thermo-sensitive period of eui gene. And this change of fertility was as same as Peiai 64S. So the expression of eui gene could not effect the fertility of Changxuan 3S.