Studies On Tissue Culture And Tetraploid Induction Of Lily

In this paper we studied tissue culture of Lily systematically , established a good diploid system, used radiation mutagenesis and colchicines liquid treatments to induce tetraploid plants, in order to offer significant referenced datum for the future. The results showed:1, There were different frequencies of buds induced and differentiation with different genotype varieties, the frequency of buds differentiation with Lilium davidii var. unicalar Cotton was 51.65%, the average of induced bulblets was 5.87; that of Lilium brownii E E. Brown var. viridulum Baker was 22.22% and 2.15.2, Different part of a bud scale gave different responses to the effect of induction with the same varieties. The inducting frequencies of inner scales with Lilium davidii var. unicalar Cotton was the highest, middle scales was the second ,and outer scales was the lowest; But the result of Lilium brownii F. E. Brown var. viridulum Baker was quite the contrary.3, There were different hormone categories and concentration combinations in leaves' ability of induction and differentiation, when the concentration range of 2, 4-D was 1.5-2.5mg.L ' ,it's good for callus induction; when the medium combine with 0.5 mg.L-16-BA and 0.5 mg.L-1NAA were more profit for bud induction.4 , The optimal hormone combination for embryoids multiplication of Lilium longiflorum was 0.5mg.L-1KT and 0.5 mg.L-16-BA.5, Choosing one-factor auxin was adverse to buds subculture multiplication of Lily, for example NAA or IAA.6, Low concentration of KT was of advantage to buds subculture multiplication of Lilium davidii var. unicalar Cotton; and higher concentration of 6-BA was better to Lilium longiflorum's multiplication.7, Lower concentration range of MET (0.3-0.6mg.L-1) can accelerate budssubculture multiplication of lily.8, Different hormone combinations gave different responses to the effect of buds subculture multiplication. The treatment with BA0.5mg.L-1+NAA0.1mg.L-1+MET o.5mg.L-1+KTo.5 mg.L"1 showed better effect than others.9, The medium combine with two auxins were more propitious to root inducing and sound seedling of Lily than that of one-factor auxin.10 , Lower concentration range of MET(0.6-0.8mg.L-1) can accelerate root inducing and sound seedling of Lily.11, The best effect concentration of ABT1 on root inducing and sound seedling with Lilium davidii var. unicalar Cotton was 2.0 mg.L-1.12, There were different radiation sensitivity with different genotype varieties. The sensitivity of Lilium davidii var. unicalar Cotton was better than Lilium longiflorum.13 , The effects of different concentrations and time of colchicines liquid treatment with different genotype varieties. Best treatment for inducing frequency was obtained when the concentration of colchicines was 0.7% for 5 days, the inducting frequency of Lilium davidii var. unicalar Cotton being 40%, and Lilium longiflorum being 30%.14, The effects of mutagenesis on different genotype varieties combined with different colchicines liquid treatment. Lilium davidii var. unicalar Cotton was suit of soaking with colchicines, and Lilium longiflorum was suit of embedding with absorbent cotton.15, Comparative observations on leaf stoma's numbers of guard cell and size between tetraploid and diploid of Lily showed significant difference.16 , Morphological observation showed that the chromosome numbers of diploid Lilium davidii var. unicalar Cotton and Lilium longiflorum was 24(2n=2X=24,X=12), while that of tetraploid plants was 48(4n=4X=48,X=24).