| Lily posses the better economical value, is a kind of flower, acting on ornamental, edible and officinal function. And it is the both ornamental and edible plant examined and approved by department of sanitation firstly. Accordingly, In the study, based on the previous research, the systemic tissue culture for the best rapid mode propagation is studied, which can be used for industrial production of the lily on a great scale and fine lily genus abroad rapid propagation and genus improvement; Meanwhile, combining with chromosome engineering, the method and technology on selectively breeding lily germplasm is done for obtaining the optional breeding method.The effective system in vitro regeneration of the lilium davidii var. Unicolor (hoog)Cotton and Lilium davidii Duchautre from yangling of Shanxi is built. And It is done systematically that filtration of sterilizing explants , selecting the type of the explants, sieving the culture medium, and the transplanting and rooting of the tissue plantlets. And the bulb of the lily in the tube is induced successfully, further to compensate the deficiency of tissue culture, and it provides a new method for rapid propagation of lily.The result in the culture in vitro of the two kinds of lily shows that obtained sterile explants is the critical step in plant tissue culture. As a result, the explants sterilizing and the inducing rate of bud affect directly the experiment process. The best sterile method is that 10 min immersing by scour, 30 min scouring by water, 120 min sterilizing by 75% ethanol, 12 min sterilizing by 0.1% hydrargyrum, rinsing 4-6 times by sterilized water. The induced rate is 90%, and the polluted rate is only 2%. The time of shoot formation is 20-27 days, and the result show that the appropriate medium for inducing the differentiation on bud of the Lilium davidii Duchautre is MS+6-BA 1 .0mg/1 +NAA0.5mg/L + sucrose 3%; The appropriate medium for inducing the differentiation on bud of the Lilium davidii var. Unicolor(hoog)Cotton is MS + 6-BA 1 .0mg/1 + MAA 0.2mg/L + sucrose 3%. As for the Lilium davidii Duchautre, the appropriate medium on differentiation and propagation of bud is 6-BA1.0mg/l +NAA0.5mg/L+ sucrose 5%; As for the Lilium davidii var.Unicolor(hoog)Cotton . the appropriate medium on step-generation and propagation of bud is 6-BA0.5mg/l +NAA0.1mg/L+sucrose5%. The result shows that it is the best that lily explants is squama and the base of squama is the better. As for the Lilium davidii var. Unicolor(hoog)Cotton , the optimal medium induced to form the bulb of lily is l/2MS+IBA0.5mg/L; As for Lilium davidii Duchautre, the optimal medium induced to form the bulb of lily is 1/2MS+IAA1.0mg/L the position formed the bulb of the both Lily differed, Lilium davidii var .Unicolor(hoog)Cotton form in the base for more than 3.5 on the average, the Lilium davidii Duchautre form in the top of the cultured plant and the every plant only form one bulb. It is easy to root comparatively, the both optimal medium for rooting is also 1/2MS+IBA0.5mg/L, the rate of rooting is 100%, the number of roots produced by everyplant is more than 8.2. Lilium davidii vor.Unicolor(hoog)Cotton is 8.0.On the base of building the system of regeneration in vitro, The inducing tetraploids of the Lilium davidii var. Unicolor(hoog)Cotton is done by the solution of colchicines. Two methods were adapted: marinating and composite culture. The marinating individe further the marinating of tube seeding and the marinating of bulb. The result show: the marinating is a better method and the marinating of the tube seeding is the best. The autoployploid could be induced by immersed the stems with buds in 0.05% colchicines solution for 25 hours that is prior to culture. The induced seeding is identified by the number of the polyploidy. The number of the chromosome is 2n=4x=48, along to tetraploids plaats. The highest rate of inducing is 40.5%.Plant morphology and cytology observation on two ploidy of Lillian davidii vor.Unicolor . (hoog)Cotton with similar growth stage and en... |
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