| Shatian pomelo(Cilrus grandis var. shatianyou Hort)is a subtropical evengreen fruit known for its delicious flavor. While too many seeds in the fruit effects its edible character and commercial character in some extent. Alloteraploid somatic hybrid (provided by Pro,D.X.X) were cross-pollinated with pomelo for seed abortion. The hybrid embryo coming from the sexual hybridization (crossed between tetraploid and diploid) was highly sterile. While new triploid germplasm in Citrus were obtained, with the technique of embryo rescue. The main study results as follows:1 Comparing the abortive seed fruits with Con, I Seed of cross-pollinated fruits were significantly found to be abortive; II The weight, size, total sugar content, and solubility of solid substance of fruit were less than that of Con, but no significant difference ; III The percentage of edible, sour and Vc were higher than that of Con, but no significant difference as well; IV The suitable allotetraploid somatic hybrids for seed abortion were HR and SR2 It was a useful way to apply the orthogonal design in the pomelo tissue culture. The results showed that the optimal medium for embryo rescue was solid MT medium supplemented with GA31mgL-1, LH40mgL-1 and queen bee oar 500mgL-1or solid MT medium supplemented with ME400mgL-1; The optimal medium for adventitious shoots multiplication was solid MT medium supplemented with BA0.5mgL-1, NAA0.1mgL-1 and GA30.2mgL-1; The optimal medium for tube-grafting was solid MT medium supplemented with BA0.5mgL-1; And The optimal medium for adventitious roots induction was solid MT medium supplemented with IBA3.0mgL-1, NAA1.0mgL-1 and AC0.1%.3 A total of 46 plants from 3 different combinations were obtained.Randomly selected 3 of them and examined by the apparatus of PA-1, the results showed that 2 of them were triploid plants.4 Triploid plants could also be obtained from the seed abortive mature fruit under proper conditions.
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