Constructing The Fingerprint Of S.intagra And S.suchowensis Cheng

In this paper genetic diversity and fingerprintings of S.intagra and S.suchowensis Cheng with microsatellite(SSR) markers. Based on the study researching direction of Salix were discussed. The results of follows:The samples from 78 S.intagra and 19 S.suchowensis Cheng were amplify and analysis with 23 polymorphic microsatellite loci screened from 496 Poplus primers. Average number of alleles(A) and effective number of alleles(Ne) were 4.3913 and 2.2199 in the interspecies. The mean expected heterozygosity(He) was 0.4350, the mean Nei diversity index(h) was 0.4409 and Shannon information index was 0.6800 on average.In S.intagra, average number of alleles(A) and effective number of alleles(Ne) were 3.8261 and 2.1097 and Shannon information index was 0.6800 on average in the intraspecies more than in S.suchowensis Cheng. In S.suchowensis Cheng, average number of alleles(A) and effective number of alleles(Ne) were 2.5217 and 1.6345 and Shannon information index was 0.6800 on average in the intraspecies.In 23 pairs of primers, S.intagra could identified from S.suchowensis Cheng with primer ORNL₁46. I1 I27 S.intagra had specific band in the fingerprint of the primer ORNL₃08 differing from other 51 S.intagra. I1 I27 S.intagra were identified by primer ORNL₃12, ORNL₃01, ORNL₂17, ORNL₂82, ORNL.140, ORNL.211, ORNL₄10, ORNL₁49, ORNL₆3. Another 51 S.intagra were identified by primer ORNL₃12, ORNL₃08, ORNL₂17, ORNL₃01, ORNL₁46.19 S.suchowensis Cheng were identified by primer ORNL₄64, ORNL₁40, ORNL₄09, ORNL₁26, ORNL₁22, ORNL₈5, ORNL₁49.18 S.intagra had specific band in the fingerprint of the primer ORNL₃12. 4 S.intagra had specific band in the fingerprint of the primer ORNL₄64. Primer ORNL₁46, ORNL₄64, ORNL₃12, ORNL₃08 were more efficient in constructing willow fingerprint.In 23 pairs of primers, 97 willow species could identified by the core 15 pairs of primers.According to its biological and genetic properties, Salix had significantly utilization in forest genetics. This paper researched basically S.intagra and S.suchowensis Cheng.