Effect Of Diet Composition On Absorbable Amino Acids In Goats

Four xuhuai white goats fitted with permanently rumen fistulae and proximal duodenum fistulae were used in a 4 X 4 latin square design to determine the effects of different diets composition to the absorbable amino acids of goat with different SC (structure carbohydrate) /CP(crude protein)(SC/CP were 3.62, 2.95, 2.29 and 1.65 respectively), and use DAPA and PC respectively as internal marker of rumen bacteria and protozoa to measure the production of bacteria and protozoa of entering the duodenum and flux and composition of all kinds of amino acids of digested diet bypass-protein-N from rumen. And at the same time, the dynamic changing rules of ruminal and duodenum internal enviroment parameters were sturdied. The results showed that:1. The hightest output of total bacteria amino acid observed in the B group was 1.57g/d. The net absorbable amino acid of bacteria was 1.54g/d in B group, much higher than in the others. And the contribution proportion of bacteria amino acid to the total absorbable amino acid in duodenum in B group was higher than in the others significantly. There have no significant differentce among 17 amino acids between LAB and PAB except Thr, Met, Leu and Lys.2. The protozoa amino acid to duodenum in A group was 1.25g/d, much higher than in the others. And the output of protozoa amino acid in D group much lower than in the others. Different diet structure showed significant effect on Glu, Arg, Tyr, Pro and Ala of rumen protozoa.3. The total amino acid in B group was much higher than in the others. The by-passed rumen diet amino acid in C group was 2.03g/d, much higher in than others. As contribution proportion is concerned, the proportion of B group was 42%, lower thanin the others. The proportion of C group was 62%, much higher than the others. Almost of all AA output increases to the highest point after eating 6 hours. There were significant difference among 11 amino acid out of 17 amino acid.4.With the SC/CP decreasing, nominal pH decreased significantly. pH decreased from 6.37 in A group to 5.86 in D group. pH in A group was higher than in the B, C, D group significantly(P<0.01). pH in duodenum were 3.40,3.52,3.23 and 3.58 in A, B, C, D group respectively. pH in C group was lower than in D group significantly.5. Lower SC/CP increased ammonia-N (NHs-N) concentration increased from 8.37 in A group to 13.71 mg/100ml in D group. B, C, D groups increased NH3-N concentration to a large extent compared with A group(P<0.01). NHa-N concentration in B group was lower than in the others significantly in duodenum.