Font Size: a A A

Study On Genetic Diversity Of Pinus Sibirica Du Tour With ISSR-PCR

Posted on:2005-08-24Degree:MasterType:Thesis
Country:ChinaCandidate:L WeiFull Text:PDF
GTID:2133360125453468Subject:Tree genetics and breeding
Abstract/Summary:PDF Full Text Request
In this article, we adopt data from variation characters of phenotype of needles and data from variation character on molecule level, which both base on the 19 populations (six populations of Xisayan-Altai ecotype district, five populations of south altiplano ecotype district in East-Siberia and eight populations of plain ecotype district in West-Siberia) of an introduce, Pinus sibirica Du Tour in Russia in different geographical areas, based on theories of population genetics, biosystem, ecobotany and geobotany to partition groups of Pinus sibirica Du Tour and its genetic diversity by means of multi-statistics and ISSR-PCR. The following results are worked out: 1.Results for genetic diversity and differentiation in heredity of Pinus sibirica Du Tour(1) 147 sites of twelve primers were assured by ISSR-PCR and there are 135 in total. Ratio of multiform sites (11.25 sites in average) of total to 91.84%. Ratio of multiform sites of populations amount to27.07% in average. Index Nei is 0.4131, index Shannon is 0.2665, Gst (0.3807 in the average) has a large range of alteration, Nm is 0.8134;Gene diversity (of all the gene diversity of populations) within populations account for 61.93%, gene diversity (of all the gene diversity of populations) between populations account for 38.07%; These show that Pinus sibirica Du Tour has abundant genetic diversity of populations and great genetic dominance on introduction and breeding.(2) Multiform sites' ratio among the three ecotype district has a rule as follows: H (Xisayan-Altai ecotype district) > J(south altiplano ecotype district in East-Siberia) > W(plain ecotype district in West-Siberia). Index Shannon is 0.4079 in the average, H(0.4143 )> W(0.3204)> J( 0.3260) .Gene diversity within series account for 86.33% of the total, gene diversity between the three series account for 13.67% of the whole groups, Nm is 3.1567, the average genetic distance is 0.0754, the genetic identify is 0.9274.(3) Multiform sites' ratio of Xisayan-Altai ecotype district is 81.63%, the average multiform sites' ratio of populations is 32.00%. Index Nei is 0.2628, index Shannon is 0.4007.Multiform sites decline according to the ascent of altitude, lower altitude has more multiform sites. Nm is 0.6335, the average Gst is 0.4411;gene diversity within populations account for 55.89% of the total, gene diversity between populations account for 44.11% of total. The genetic identify is 0.8090, the average geneticdistance is 0.2153.(4) Multiform sites' ratio of south altiplano ecotype district in East-Siberia is 68.03%, the average multiform rat: of populations is 25.71.%, index Nei is 0.1976,index Shannon is 0.3048, the average Ht (gene diversity of the whole populations) is 0.1983, the average Hs (gene diversity within the groups) is 0.1006: Nm is 0.5148, the average Gst is 0.4927;Gene diversitv within populations account for 50.73% of the total; gene diversity between groups account for 49.27% of total. The genetic identify is 0.8640, the average genetic distance is 0.1495.(5) Multiform sites' ratio of the plain ecotype district in West-Siberia is 65.99%, the average multiform sites'ratioun populations is 2-4.06%, index Shannon is 0.3132,index Nei is 0.2062, the average Ht (gene diversity of the whole groups) 's 0.2059, the average Hs (gene diversity within the groups) is 0.1091 ; Nm is 0.5636, the average Gst is 0.4701; gene diversity within populations account for 52.99% of all the groups, gene diversity between groups account for 47.01% of total;The average genetic distance is 0.1487, the genetic identify is 0.8627. 2. Result of dividing populationsAfter synthesizing the progress of clustering analysis, rule of geographical variation and Russiau scholars' study, as a whole, the seven primary allocation zones of seeds (nineteen populations) can be divided into three ecotype district of populations zones according to main ecological factors, that is Xisayan-Altai ecotype district, south altiplano ecotype district in East-Siberia and plain ecotype district in West-Siberia. Populat...
Keywords/Search Tags:Pinus sibirica Du Tour, ISSR, genetic diversity, dividing populations, Xisayan-Altai ecotype district, South altiplano ecotype district in East-Siberia, Plain ecotype district in West-Siberia
PDF Full Text Request
Related items