Studies On Biological Effect Of Low Energy Ion Beam In Wheat

Using Wei6 Yunong92349 andYumai 52 as materials, mutagenic effect of low energy nitrogen ion implantation and biological effect of ion beam-mediated total DNA transformation were studied.In study on mutagenic effect of ion implantation, we found there were aberrations of M1 generation in several aspects. Firstly, the germination rate-dose effect curve was special "saddle shape". Secondly, when the dose was changed, the physiological damage rates of the nutrient organs were different, including the height of the plants, the coleoptiles and the length of the leaves. One of the most evident aspects was the short of the length of the first leaf of wheat. Moreover, it was found that the coleoptile of Wen6 became purple with the ion beam implantation. Lastly, with the analysis of the peroxidase enzyme and sterase isozyme spectrum of wheat leaves, the result showed that the number of the isoenzyme bands were not obviously different, but with clearly different activity.During the study on ion beam-mediated exogenous DNA transformation, isozyme spectrum, soluble protein chromatograms of Seeds and the genomic DNA polymorphic bands by RAPD method was analyzed.Seeds of male fertile plants and cross-bred seeds of dwarfing male sterile plants from transformation lines 9002 and 9004 and seeds of the acceptor Yumai 52 were analyzed by comparison of their polyacrylamide gel electrophoretic patterns of soluble proteins and esterase isozyme. The results showed that the soluble protein chromatograms of Seeds of transformed plants were different from those of seeds of the acceptor. One of the most evident aspects was that some bands were lost. In addition, it was found that some bands was more deep-dyed in transformation lines 9004. This result indicated that its total seed protein content is higher. Evident differences on esterase isozyme and soluble proteins chromatograms of cross-bred seeds of male sterile plants were observed . It may be concluded that some soybeanDNA fragments were inserted into the genome of the acceptor as the result of introducing exogenous DNA, which would consequently lead to the mutation of some acceptor genes or change the gene expression.Using RAPD marker, the results showed that five out of twenty-four primers yielded DNA bands of the transferred progenies which were different from the acceptor, which were shown by the number and the staining degree of bands. Moreover, there were the difference in DNA bands between two transformation lines. Primer S164 yielded a clear new band. In addition, in the F1 hybrids of sterile transformed plants, the pollen fertility and seed set in bagged panicles was observed. The results showed that there were a few sterile plants in the F1 population. The possible explanation for this result was that these male sterile plants were recessive male sterile materials.