Ion Beam Implantation And Mediated Transfer Of Exogenous Dna Into Wheat Preliminary Study

Firstly, the technology of mutation breeding and the development and application of low energy ion biology were reviewed, the situation of wheat production and its developing trend were discussed, as well as the present breeding methods. Especially the mutagenic effects of ion beam on wheat and the work of transferring the DNA of soy to wheat by ion were studied.It is shown that with the dose increase of ion beam, the ratio of young plant decreases gradually, the effects under different doses are remarkable, the sensitivity among breeds varies notably. In dose experiment, the autumn behavior of wheat indicates, the diversity among breeds, doses and the interaction between breeds and doses arrives at a prominent level, hereby it provides an appropriate dose range(5 X 1017N+/cm2~6X1017N+cm2). Many lines has been selected and obtained from implantation progenies, also the mutation types from ion implantation on wheat has been analyzed. To sum up, the Ml breeds from ion implantation appear heritable variation frequently, and the selected plants separate rarely at M2 and stabilize at M3, so that the breeding cycle has been shortened, the breeding efficiency has been enhanced.A rapid, effective and non-destructive analytical system of wheat protein has been established by near infrared system firstly. It is found that many high protein plants appear in Ml generation by means of introducing soy DNA to wheat. The highest content of protein is up to 22.83%, the mechanism of which has been discussed primarily.