The Flower Developing, Fast Propagations System Established And Study Of Flower Color Gene Polymorphism In Saliva Splendens

In this study,I determined the cause of affecting fecundity of salvia splendens,the best system of tissue culture fast propagations system and clarified flower color gene Ss5MaT1 polymorphism, through studying on the embryological structure in flower delevoping, the way of tissue culture fast propagations and flower color gene by PCR-SSCP combining with sequencing .The result of flower delevoping showed that the flower bud morphological differentiation began at 4th leaf burl and finished at 9th leaf burl, and the wall of anther was dicotyls type. Moreover, mature microspore was three-cell type, and the delevoping payway of megaspore was polygonum type, which testified that the seeding rate low wasn't due to transmissibility male gametophyte and female gametophyte failing growth.The results of observating the number of seedings in different color breed indicated the averge seeding rate in Saliva splendens was 15.81%, and the averge drop flower rate was 23.13%. the mature ovule failing growth rate was 84.1%.The best explant in the culture was stem segment with bud, and the best medium of buds inducing was 1/2MS+NAA0.1mg/L+6-BA2.0mg/L+IBA 0.5mg/L, differentiation rate was 71.4%, buds multiplication rate was 140.62%. The best of rooting medium was 1/4MS+NAA 0.5mg/L, taking root rate was 91.6%.According to the sequences of Ss5MaTI in GenBank, I obtained 916bp Ss5MaT1gene partial cDNA sequence of eigh species by RT-PCR.According to cDNA sequence that obtained in RT-PCR, four pairs of primers were designed. Aim segment was amplificated throgh nested PCR. PCR-SSCP technology was applied to detect SNPs of the Ss5MaT1. Detected results demonstrate that only one pair primer amplification segment had polymorphism, but others had not been different. The result of sequening showed that the cDNA segment that was amlificated by RT-PCR had high homology, only the Salmon and the rose had a mutation point respectively. Rose: 744 C T, Ile Thr; Salmon: 678 C T, Val Ala, and others had not mutation point. The cloning and sequening result was identical to the result of PCR-SSCP, which showed PCR-SSCP could examine the mutation of flower gene. Master Candidate:Li Fenglang Speciality: botany Advisor: Professor Hu Baozhong...