Analysis Of Resistant Gene Against Cercospora Sojina Race 1 In Soybean With Molecular Markers And Germplasm Identification

Cercospora sojina Hara occurs worldwide and is one of major soybean disease in northeast of China, harming soybean seriously. The most effective and economical way is planting resistant cultivars, so, breeding resistant cultivar is important by finding resistant gene location, which with long-time and more -races resistance to Cercospora sojina Hara.In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, Bulked segregant analysis was applied to F5 populations derived from cross between the resistant line DongNong40566 and the susceptible line DongNong410, self-crossing for three generations after F2. The resistant and susceptible proportion show that Cercospora sojina Hara was resistant by single gene . three SSR markers including Satt565, SOYGPATR and Satt396 were identified from 500 SSR markers, the linkage order is Satt565 SOYGPATRHrcslSatt396, and the distance to the resistance locus is 12.7,6.5> 14.7cM respectively. These three markers and the resistance locus were mapped to soybean linkage group C1.Three SSR Markers Satt565, SOYGPATR and Satt396 were identified by 45 resistant and susceptible lines, result show that allele SOYGPATR-2, SOYGPATR-3 and Satt396-l accord with resistant and susceptible trait highly. They can identify resistant cultivars with81.8%> 85.7% > 72.7% probability. So, they have some value in resistance source screening.