Effects Of Amino Acid Trace Mineral Chelates On Performance And Physiological Function Of Laying Hens

Three trials were conducted to study the effects of amino acid trace mineral chelates(AATMC) on performance and physiological function of laying hens at peak period . 1440 160-day-old Rohman laying hens were divided into three experiments randomly with four replicates per treatment and thirty hens per replicate. The basal diet was corn-soybean mealtype that the level of Fe, Cu, Mn, Zn, Se and I was 60, 20,100, 80, 0.3 and 0.4 mg/kg respectively in FeSO4 ,CuSO4 ,MnSO4 ,ZnSO4 ,Na2SeO3 and KI. In trial 1, five treatments were arranged to investigate the influence of substituting the mixture of iron methionine (Fe-Met), copper methionine (Cu-Met), zinc methionine (Zn-Met), manganese methionine (Mn-Met) and selenium methionine (Se-Met) for the mixture of corresponsive inorganic microelement sulfate with different level of 0%, 20%, 50%, 80%, and 100%. In trial 2, 2X2 factorial arrangement of treatments was employed to evaluate the effects of substituting Zn-Met and Mn-Met for zinc sulfate and manganese sulfate with different level of 50% and 100% and was compared to the basal diet with Zn and Mn sulfate. The total number of treatments was five. In trial 3, six treatments were arranged to investigate the effects of methionine mineral chalets on the same methionine level in the diet by deducting or not deducting the amount of methionine in chelates from basal diet. Six treatments were conducted : (1)basal diet (B); (2) B+0.04675%DL-Met; (3)B-0.04675% Met+mixture of AATMC substituted for mixture of inorganic microelement with 100% level; (4) B + mixture of AATMC substituted for mixture of inorganic microelement with 100% level; (5) B-0.0234% Met+mixture of AATMC substituted for mixture inorganic microelement with 50% level; (6) B+mixture of AATMC substituted for mixture of inorganic microelementwith 50% level.The results of trial 1 were: the performance, physiological function and egg quality of laying hens could be improved by substituting mixture of AATMC for mixture of inorganic salt (P>0.05) with the rate of broken egg reduced and the activity of Alkaline Phosphatase (AKP) increased significantly (P<0.01), the content of IgG, IgM and the rate of proliferation of T lymphocyte increased significantly (P<0.05).The lowest of feed consumption and feed consumption per kg egg production was obtained in the treatment 3 of 50% substitute (P<0.05). The optimum level of substitution of AATMC was 50%.The results of Trial 2 were: The performance of laying hens was tended to improve by substituted Zn-Met and Mn-Met for Zn sulfate and Mn sulfate with the largest improvement in treatment of Zn-Met50%\Mn-Metl00%, then the treatment of Zn-Metl00%\Mn-Metl00%.The performance of laying hens was no difference significantly between the substitute level of 50% and 100% of Zn-Met, but was tend to be better in substitute level of 100% of Mn-Met than in 50% of Mn-Met with the feed consumption per kg egg production significant.(P<0.05) There were no significantly interaction between Zn-Met and Mn-Me onperformance of laying hens. The content of serum Zn, activity of AKP and LDH, and the humoral immune responses could be improved significantly by substituted Zn-Met/Mn-Met in level of 50%/100% (P<0.05). According to egg production and physiological indexes of laying hens, the optimum substitute level of Zn-Met\Mn-Met was 50%\100%. Compared with the results of Trial 1, the performance of laying hens were better by using mixture of AATMC man using Zn-Met\Mn-Met when substitute level was 50%, and the results were adverse when substitute level was 100% The results of Trial 3 were: At the same level of Met in the diet, the performance and immune function of laying hens, egg shell quality were be improved by using the mixture of AATMCWhen substitute level of AATMC was 100%, the performance of laying hens was better in treatment of Met-deducted from diet than that of Met-no- deducted. When substitute level of AATMC was 50%, the performance of laying hens was not different between the two treatments of Met-deducted and Met-no- deducted. These results imp...