| The experiment was conducted to investigate effects of feeding different dietary crude protein and calcium levels during the overall reproduce time on immunity, reproductive function and blood physiological and biochemical indexes of breeder roosters. 135 New Yangzhou breeder roosters at 21-week-old were sampled, and randomly divided into 9 groups of 15 roosters each, fed with 9 diets designed by a 2 X 3 factorial arrangement of three crude protein levels (17%, 14%, 11%) and three calcium levels (3.3%, 2.3%, 1.3%) with the same other nutrition levels. The results showed that:l.At the beginning of the experiment, there was an evident uptrend of growth in roosters. Body weight of groups which were fed with the diet of high protein and low calcium well. But there was no significant change of each group in the later of the experiment. Body weight of aged roosters was not affected by the different dietary of crude protein and calcium levels (P>0.05) .2. Serum antibody titre of the roosters of each group was on the increase with various degrees after injection. Serum antibody titre of the roosters fed the low protein and low calcium diets increased rapidly during 15 days and 30 days after injection, and decreased evidently after 90 days and 135 days than that of the other groups (P<0.01) . Compared with the group that used high protein, high calcium, the ND antibody titre of the roosters receiving low protein, low calcium were significantly higher (P<0.05) . Crude protein and calcium had significant influence on the serum antibody titre to ND (P<0.05 ) , and the interaction of which did not affect it significantly (P>0.05) .3.Croud protein had more influence on sperm quality than calcium and interaction of the two factors. Sperm quantity, sperm motility, sperm concentration of the roosters which were used low protein, low calcium diet were significantly higher than those of the group of fed high protein, high calcium diet( P>0.05 ),but available sperm significant(P<0.05), and sperm abnormality was lower conversely (P>.05). Weight, cubage and index of spermary of trial roosters were lower significantly in high calcium treatment than those in low calcium treatment (P<0.05) . From 46 weeks old to 70 weeks old, weight, cubage and index of spermary of trial roosters were shrink slowly. The interaction of crude protein and calcium had significant effect on serum testosterone(T)(P<0.05), and had higher significant influence on luteinizing hormone(LH) (P<0.01). Sperm quantity had a positive correlation with sperm concentration(P<0.05). There was a positive correlation between sperm motility and available sperm, follicule stimulating hormone(FSH) (PO.05 ) , and between available sperm and FSH, left spermary index. FSH had a strong positive correlation with spermary index (PO.01 ) .4.Albumin content of feeding low protein diet was more significantly than middling protein (PO.05 ) . The content of total protein and globulin of the group that used low calcium diet were significantly higher than the group feeding high calcium diet (PO.05) . Under a certain level of phosphorus of each diet, serum calcium and phosphorus were comparatively balanced with the increasing of the dietary calcium level. The content of serum calcium approached about 16.0mg/dl, and the serum phosphorus was around 3.5mg/dl. The activity of ALP of the group that fed low calcium diet was higher than that of other groups (P>0.05 ) .5.GLU content of roosters feeding low protein diet was slightly higher than the other two groups (P>0.05) . The content of TG of the group fed low protein diet was significantly higher than high protein group (PO.01) . The content of the group that used high protein diet was significantly higher than the other two groups (PO.01). The activity of GPT of the group fed high protein was highest, but no significant influence(P>0.05) . GOT activity of roosters feeding high protein was significantly higher than middling protein (PO.01) . Compared to the group that used high protein diet, and corticoids in the group fed low protein...
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