Studies On The Function And Activation Of Honeybee Drone Larvae

Drone larvae of ten days age are not only a kind of good nourishments but also a kind of good substance for bee therapy and health care. Firstly, I had test the accurate content of water in drone larvae in order to confirm the accurate dosage. Secondly, I took their safety as food into experiment. Thirdly, I did many experiments on their function of anti-fatigue and anti-hyperirritability and immunity enhancement. Through these experiments I had figured out the best dosage for these uses. I also found the combination drone larvae and Chinese herbal medicine can make their function repaired with each other. I experimented out the best ratio between them and the best dosage of the mixture inwhich contains 30 percent Chinese herbal medicine.Safety as food experiments: Acute toxin experiments, four dosage were selected: 10. Og/kg, 21. 5g/kg, 46. 4g/kg and lOOg/kg, giving them to mice within 24hours, 12hours of feeding prohibition of them before experiments. The reaction and the death information of the mice were observed. The result shows that drone larvae and Chinese herbal medicine and their mixture have no acute negative effect to mice. Inheritance toxin experiments, Chinese herbal medicine is not safe as drone larvae and their mixture. But it is toxin only when the dosage is as high as 20. Og/kg. All these demonstrate that drone larvae and Chinese herbal medicine and their mixture are safety enough as nourishments.The time of swim, the time of climbing pole, lactic acid of blood, blood serum urea nitrogen, lactic acid dehydrogenation enzyme, glycogen of liver are investigated to appraise anti-fatigue. Result shows drone larvae are good on anti-fatigue and low dosage such as 0. 25g/kg also hasthe effect on anti-fatigue. The best dosage is 3. 65g/kg. The death timewhen mice were placed in high or low temperature conditions were used to measure the effect of drone larvae on anti-hyperirritability. The best dosage is 3. 8g/kg. The time of swim was investigated to appraise anti-fatigue of the mixture that made of drone larvae and Chinese herbal medicine. Result shows when Chinese herbal medicine has 36. 2 percent in the mixture can make drone larvae have the best function on anti-fatigue. The best dosage is 3. 06g/kg.The weight of immunity apparatus, experiment of phagocytosis of macyophage , DTH and the value of blood serum hemolysin were investigated to appraise Nonspecific immunity, Cellular immunity and Humoral immunity. Result shows that drone larvae can markedly enhance immunity function in mice. They have effect even with low dosage and the best dosage is 3. 62g/kg. DTH was investigated to appraise Cellular immunity of drone larvae and Chinese herbal medicine' s mixture. Result shows when Chinese herbal medicine has 29. 7 percent in the mixture can enhance their immunity function the best and the best dosage is 3. 08 g/kg.The experiments in finding the best ratio of drone larvae and Chinese herbal medicine show that the Chinese herbal medicine can badly enhance drone larvae' s effect on anti-fatigue and immunity function in mice. The result is better than only using drone larvae when the mixture contains 20%-50% Chinese herbal medicine in the experiment of mice' s swim. In the case of DTH the percentage of Chinese herbal medicine is 20%-30%.Correlation analysis shows there has a good relativity between the time of swim and the other anti-fatigue indexes. DTH and the other immunity indexes also have good relativities.